TIMP1 Recombinant Rabbit Monoclonal Antibody [PSH21-35]
cat.: HA751798
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH21-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within rat TIMP1 aa 1-217. |
| Positive control: | C2C12 cell lysate, MEF cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P12032 Mouse |
| Alternative names: | Clgi Collagenase inhibitor EPA EPO Erythroid Potentiating Activity Erythroid-potentiating activity Fibroblast collagenase inhibitor FLJ90373 HCI Human Collagenase Inhibitor Metalloproteinase inhibitor 1 Metalloproteinase inhibitor 1 precursor OTTHUMP00000023214 TIMP 1 TIMP TIMP metallopeptidase inhibitor 1 TIMP-1 Timp1 TIMP1 protein Tissue Inhibitor of Metalloproteinase 1 Tissue inhibitor of metalloproteinases 1 Tissue inhibitor of metalloproteinases Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor |
Images
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Fig1:
Western blot analysis of TIMP1 on different lysates with Rabbit anti-TIMP1 antibody (HA751798) at 1/5,000 dilution. Lane 1: C2C12 cell lysate Lane 2: Neuro-2a cell lysate (negative) Lane 3: MEF cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751798) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.