FPR2 Recombinant Rabbit Monoclonal Antibody [PSH21-63]
cat.: HA751802
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH21-63 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human FPR2 aa 1-50. |
| Positive control: | HL-60 cell lysate, THP-1 cell lysate, J774A.1 cell lysate, RAW264.7 cell lysate, Mouse brain tissue lysate, Mouse bone marrow tissue lysate, Rat brain tissue lysate, THP-1. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: P25090 Human | O88536 Mouse Entrez Gene: 690158 Rat |
| Alternative names: | ALXR FMLP-R-I FMLP-R-II FMLP-related receptor I FMLPX Formyl peptide receptor 2 Formyl peptide receptor related Formyl peptide receptor-like 1 FPR2 FPR2_HUMAN FPR2A FPRH1 FPRH2 FPRL1 HM63 Lipoxin A4 receptor LXA4 receptor LXA4R N-formyl peptide receptor 2 RFP |
Images
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Fig1:
Western blot analysis of FPR2 on different lysates with Rabbit anti-FPR2 antibody (HA751802) at 1/5,000 dilution. Lane 1: HL-60 cell lysate (20 µg/Lane) Lane 2: THP-1 cell lysate (20 µg/Lane) Lane 3: J774A.1 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Mouse bone marrow tissue lysate (40 µg/Lane) Lane 7: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 39 kDa Observed band size: 35 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751802) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of THP-1 cells labeling FPR2 with Rabbit anti-FPR2 antibody (HA751802) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FPR2 antibody (HA751802) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of THP-1 cells labeling FPR2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751802, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.