NDUFV1 Recombinant Rabbit Monoclonal Antibody [PSH21-97]
cat.: HA751813
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Monkey, Pig
Applications: WB, IF-Cell, IHC-P, IP
Clonality: Monoclonal
Clone number: PSH21-97
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Recombinant protein within human NDUFV1 aa 1-464.
Positive control: A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, COS-1 cell lysate, 4T1 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Rat heart tissue lysate, Pig brain tissue lysate, Pig spleen tissue lysate, A549, C2C12, PC-12, human colorectal carcinoma tissue, human heart tissue, mouse heart tissue.
Subcellular location: Mitochondrion inner membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IP
1:5,000
1:50
1:1,000
1-2μg/sample
Uniprot #: SwissProt: P49821 Human | Q91YT0 Mouse
Entrez Gene: 293655 Rat
Alternative names: CI 51kD CI-51kD CI51KD Complex I 51kD Complex I-51kD FLJ59059 mitochondrial NADH dehydrogenase (ubiquinone) flavoprotein 1 NADH dehydrogenase [ubiquinone] flavoprotein 1 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial NADH dehydrogenase flavoprotein 1 NADH ubiquinone oxidoreductase 51 kDa subunit NADH ubiquinone oxidoreductase NADH ubiquinone oxidoreductase core subunit V1 NADH-ubiquinone oxidoreductase 51 kDa subunit NDUFV 1 ndufv1 NDUV1_HUMAN UQOR1
Images
HA751813_1.jpg Fig1: Western blot analysis of NDUFV1 on different lysates with Rabbit anti-NDUFV1 antibody (HA751813) at 1/5,000 dilution.

Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: COS-1 cell lysate (20 µg/Lane)
Lane 5: 4T1 cell lysate (20 µg/Lane)
Lane 6: C2C12 cell lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (20 µg/Lane)

Predicted band size: 51 kDa
Observed band size: 49 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751813) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA751813_2.jpg Fig2: Western blot analysis of NDUFV1 on different lysates with Rabbit anti-NDUFV1 antibody (HA751813) at 1/5,000 dilution.

Lane 1: Mouse heart tissue lysate (20 µg/Lane)
Lane 2: Mouse kidney tissue lysate (20 µg/Lane)
Lane 3: Rat heart tissue lysate (20 µg/Lane)
Lane 4: Pig brain tissue lysate (20 µg/Lane)
Lane 5: Pig spleen tissue lysate (20 µg/Lane)

Predicted band size: 51 kDa
Observed band size: 49 kDa

Exposure time: Lane 1-3: 10 seconds; Lane 4-5: 1 minute 33 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751813) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA751813_3.jpg Fig3: Immunocytochemistry analysis of A549 cells labeling NDUFV1 with Rabbit anti-NDUFV1 antibody (HA751813) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NDUFV1 antibody (HA751813) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA751813_4.jpg Fig4: Immunocytochemistry analysis of C2C12 cells labeling NDUFV1 with Rabbit anti-NDUFV1 antibody (HA751813) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NDUFV1 antibody (HA751813) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA751813_5.jpg Fig5: Immunocytochemistry analysis of PC-12 cells labeling NDUFV1 with Rabbit anti-NDUFV1 antibody (HA751813) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NDUFV1 antibody (HA751813) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA751813_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma tissue with Rabbit anti-NDUFV1 antibody (HA751813) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751813) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA751813_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-NDUFV1 antibody (HA751813) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751813) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA751813_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-NDUFV1 antibody (HA751813) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751813) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA751813_9.jpg Fig9: NDUFV1 was immunoprecipitated from 0.2 mg A549 cell lysate with HA751813 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751813 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: A549 cell lysate (input)
Lane 2: HA751813 IP in A549 cell lysate
Lane 3: Rabbit IgG instead of HA751813 in A549 cell lysate

Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 10 seconds; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.