Phospho-LATS1 (T1079) Recombinant Rabbit Monoclonal Antibody [PSH21-98]
cat.: HA751814
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH21-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr1079 of Human LATS1. |
| Positive control: | SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate, HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate, RAW264.7 treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle, microtubule organizing center, spindle pole body. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: O95835 Human | Q8BYR2 Mouse |
| Alternative names: | h-warts Large tumor suppressor homolog 1 LATS large tumor suppressor homolog 1 LATS1 LATS1_HUMAN Serine threonine protein kinase LATS1 Serine/threonine-protein kinase LATS1 WARTS WARTS protein kinase wts |
Images
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Fig1:
Western blot analysis of Phospho-LATS1 (T1079) on different lysates with Rabbit anti-Phospho-LATS1 (T1079) antibody (HA751814) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate Lane 3: HeLa starved for 3 hours cell lysate Lane 4: HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: RAW264.7 cell lysate Lane 6: RAW264.7 treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 150 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751814) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Phospho-LATS1 (T1079) was immunoprecipitated from 0.2 mg HeLa treated with 50mM Calyculin A for 3 hours cell lysate with HA751814 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751814 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa treated with 50mM Calyculin A for 3 hours cell lysate (input) Lane 2: HA751814 IP in HeLa treated with 50mM Calyculin A for 3 hours cell lysate Lane 3: Rabbit IgG instead of HA751814 in HeLa treated with 50mM Calyculin A for 3 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 34 seconds; ECL: K1801 |
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Fig3:
Western blot analysis of Phospho-LATS1 (T1079) on different lysates with Rabbit anti-Phospho-LATS1 (T1079) antibody (HA751814) at 1/2,000 dilution. Lane 1: HeLa starved for 3 hours cell lysate Lane 2: HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 150 kDa Exposure time: 1 minute 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751814) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.