Aurora A Recombinant Rabbit Monoclonal Antibody [PSH22-00]
cat.: HA751816
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH22-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Aurora A aa 1-150. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, BxPC-3 cell lysate, HepG2, HeLa cells treated with 100ng/mL Nocodazole for 18 hours. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Nucleus, Cell projection, Microtubule. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:1,000 1:2,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: O14965 Human |
| Alternative names: | AIK ARK-1 ARK1 AURA Aurka Aurora 2 Aurora A Aurora kinase A Aurora-related kinase 1 Aurora/IPL1 like kinase Aurora/IPL1-related kinase 1 AURORA2 Breast tumor-amplified kinase BTAK hARK1 IAK IPL1 related kinase MGC34538 OTTHUMP00000031340 OTTHUMP00000031341 OTTHUMP00000031342 OTTHUMP00000031343 OTTHUMP00000031344 OTTHUMP00000031345 OTTHUMP00000166071 OTTHUMP00000166072 PPP1R47 Protein phosphatase 1, regulatory subunit 47 Serine/threonine kinase 15 Serine/threonine kinase 6 Serine/threonine-protein kinase 15 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A STK15 STK6 STK6_HUMAN |
Images
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Fig1:
Western blot analysis of Aurora A on different lysates with Rabbit anti-Aurora A antibody (HA751816) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: Jurkat cell lysate Lane 4: HEK-293 cell lysate Lane 5: BxPC-3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751816) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling Aurora A with Rabbit anti-Aurora A antibody (HA751816) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aurora A antibody (HA751816) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa cells untreated (left) / treated with 100ng/mL Nocodazole for 18 hours (right) labeling Aurora A. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751816, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Aurora A was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA751816 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751816 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA751816 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA751816 in HepG2 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 1 minute 50 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.