Laminin beta 1 Recombinant Rabbit Monoclonal Antibody [JM099-03]
cat.: HA751844
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JM099-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 198 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Laminin beta 1 aa 1,721-1,769 / 1,786. |
| Positive control: | SW480 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate, Mouse stomach tissue lysate, Rat stomach tissue lysate, HepG2 cell lysate, Rat heart tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P07942 Human | P02469 Mouse Entrez Gene: 298941 Rat |
| Alternative names: | CLM Cutis laxa with marfanoid phenotype LAM B1 LAMB 1 LAMB1 LAMB1_HUMAN Laminin B1 Laminin B1 chain Laminin beta 1 chain Laminin beta 1 chain precursor Laminin beta1 Laminin subunit beta 1 Laminin subunit beta-1 Laminin-1 subunit beta Laminin-10 subunit beta Laminin-12 subunit beta Laminin-2 subunit beta Laminin-6 subunit beta Laminin-8 subunit beta LIS5 MGC142015 |
Images
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Fig1:
Western blot analysis of Laminin beta 1 on different lysates with Rabbit anti-Laminin beta 1 antibody (HA751844) at 1/5,000 dilution. Lane 1: SW480 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: MEF cell lysate Lane 4: C6 cell lysate Lane 5: Mouse stomach tissue lysate Lane 6: Rat stomach tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 198 kDa Observed band size: 250 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751844) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Laminin beta 1 on different lysates with Rabbit anti-Laminin beta 1 antibody (HA751844) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: C6 cell lysate (15 µg/Lane) Lane 3: Mouse stomach tissue lysate (20 µg/Lane) Lane 4: Rat heart tissue lysate (20 µg/Lane) Predicted band size: 198 kDa Observed band size: 250 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751844) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Laminin beta 1 antibody (HA751844) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751844) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Laminin beta 1 antibody (HA751844) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751844) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Laminin beta 1 antibody (HA751844) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751844) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.