S100A9 Recombinant Rabbit Monoclonal Antibody [JF096-8]
cat.: HA751845
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF096-8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human S100A9 aa 1-42 / 114. |
| Positive control: | Human cervical cancer, human breast cancer tissue, human spleen tissue. |
| Subcellular location: | Secreted, Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000 1:2,000 1:1,000-1:8,000 |
| Uniprot #: | SwissProt: P06702 Human |
| Alternative names: | Leukocyte L1 complex heavy chain 60B8AG CAGB Calgranulin B Calgranulin-B Calprotectin L1H subunit CFAG CGLB Cystic fibrosis antigen B L1AG Leukocyte L1 complex heavy chain LIAG MAC387 MIF Migration inhibitory factor related protein 14 Migration inhibitory factor-related protein 14 MRP 14 MRP-14 MRP14 Myeloid-related protein 14 NIF OTTHUMP00000015331 p14 Protein S100-A9 S100 A9 S100 calcium binding protein A9 S100 calcium binding protein A9 calgranulin B S100 calcium-binding protein A9 S100A9 S10A9_HUMAN |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-S100A9 antibody (HA751845) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751845) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-S100A9 antibody (HA751845) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751845) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Application: Immunofluorescence (IF-tissue) Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751845, 1/2,000, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig4:
Application: Immunofluorescence (IF-tissue) Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751845, 1/2,000, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.