CD130 / GP130 Recombinant Rabbit Monoclonal Antibody [PSH22-56]
cat.: HA751869
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH22-56 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CD130 / GP130 aa 1-617. |
| Positive control: | NIH/3T3 cell lysate, MEF cell lysate, Mouse thymus tissue lysate, Mouse lung tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q00560 Mouse |
| Alternative names: | CD130 CD130 antigen CDw130 gp130 GP130 RAPS IL 6R beta IL-6 receptor subunit beta IL-6R subunit beta IL-6R-beta IL-6RB IL6 ST IL6RB_HUMAN IL6ST Interleukin 6 receptor subunit beta Interleukin receptor beta chain Interleukin-6 receptor subunit beta Interleukin-6 signal transducer Membrane glycoprotein 130 Membrane glycoprotein gp130 Oncostatin M receptor Oncostatin M receptor alpha subunit Oncostatin-M receptor subunit alpha |
Images
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Fig1:
Western blot analysis of CD130 / GP130 on different lysates with Rabbit anti-CD130 / GP130 antibody (HA751869) at 1/5,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: MEF cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751869) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CD130 / GP130 on different lysates with Rabbit anti-CD130 / GP130 antibody (HA751869) at 1/5,000 dilution. Lane 1: Mouse thymus tissue lysate Lane 2: Mouse lung tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751869) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.