NK1.1 Recombinant Rabbit Monoclonal Antibody [PSH22-99]
cat.: HA751882
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IHC-Fr, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH22-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IHC-Fr IF-Cell FC |
1:500 1:500 1:500 |
| Uniprot #: | SwissProt: P27814 Mouse |
| Alternative names: | CD161 antigen like family member C CD161 antigen-like family member C CD161c Killer cell lectin-like receptor subfamily B member 1C Klrb1c Klrb1f KLRBC_MOUSE Ly-55c Ly55c Lymphocyte antigen 55c Lymphocyte antigen 59 Natural killer cell surface protein P1-40 NK1.1 NKR P1.9 NKR P1C NKR-P1 40 NKR-P1.9 NKR-P1C NKRP1 |
Images
|
Fig1:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse (C57BL/6) Tissue: Spleen Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× PBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA751882, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Mouse (C57BL/6) Sample: Spleen cell Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA751882, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
|
Fig3:
Application: Flow Cytometry
Species: Mouse Sample: C57BL/6 mouse spleen Blocking: 1% BSA + 10% normal goat serum + CD16/CD32 (Huabio), 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: mouse CD3 APC (HA720201F) and HA751882 (1/500) (Right) or Rabbit IgG Isotype Control (HA722127, left), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 594, HA1122), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.