T-bet / Tbx21 Recombinant Rabbit Monoclonal Antibody [PSH23-00]
cat.: HA751883
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH23-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:200 1:200 |
| Uniprot #: | SwissProt: Q9UL17 Human |
| Alternative names: | T bet T box 21 T box expressed in T cells T box protein 21 T box transcription factor TBX21 T cell specific T box transcription factor T cell specific T box transcription factor T bet T PET T-box protein 21 T-box transcription factor TBX21 T-cell-specific T-box transcription factor T-bet TBET TBLYM TBX 21 Tbx21 TBX21_HUMAN TPET Transcription factor TBLYM |
Images
|
Fig1:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751883, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751883, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunofluorescence (IF-tissue)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751883, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.