MCP-3/CCL7 Recombinant Rabbit Monoclonal Antibody [PSH23-04]
cat.: HA751886
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH23-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Positive control: | Jurkat (Human T-lymphoblastic cells) cell lysate, THP-1 (Human acute monoblastic leukemia cells) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P80098 Human | Q03366 Mouse |
| Alternative names: | C-C motif chemokine 7 Ccl7 CCL7_HUMAN Chemokine CC motif ligand 7 FIC MARC MCP-3 Monocyte chemoattractant protein 3 Monocyte chemotactic protein 3 NC28 RP23-350G1.4 SCYA6 SCYA7 Small-inducible cytokine A7 |
Images
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Fig1:
Western blot analysis of MCP-3/CCL7 on different lysates with Rabbit anti-MCP-3/CCL7 antibody (HA751886) at 1/5,000 dilution.
Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: THP-1 (Human acute monoblastic leukemia cells) cell lysate Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751886, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 11 kDa Observed band size: 14 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.