| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH23-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Hemopexin aa 1-462. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:20,000 |
| Uniprot #: | SwissProt: P02790 Human | Q91X72 Mouse | P20059 Rat |
| Alternative names: | Beta 1B glycoprotein Beta-1B-glycoprotein FLJ56652 HEMO HEMO_HUMAN Hemopexin Hpx HX |
|
Fig1:
Western blot analysis of Hemopexin on different lysates with Rabbit anti-Hemopexin antibody (HA751887) at 1/20,000 dilution.
Lane 1: Mouse plasma Lane 2: Mouse serum Lane 3: Rat plasma Lysates/proteins at 20 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751887, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 70 kDa |
|
Fig2:
Western blot analysis of Hemopexin on different lysates with Rabbit anti-Hemopexin antibody (HA751887) at 1/20,000 dilution.
Lane 1: Human plasma Lane 2: Human serum Lysates/proteins at 20 µg/Lane. Exposure time: 3 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751887, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 70 kDa |