MDC1 Recombinant Rabbit Monoclonal Antibody [PSH23-24]
cat.: HA751892
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH23-24 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 227 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MDC1 aa 1-150. |
| Positive control: | HeLa (Human cervical adenocarcinoma cells) cell lysate, HEK-293 (Human embryonic kidney cells) cell lysate, K-562 (Human chronic myelogenous leukemia cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, Rat testis tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:100 |
| Uniprot #: | SwissProt: Q14676 Human | Q5PSV9 Mouse | Q5U2M8 Rat |
| Alternative names: | Mediator of DNA damage checkpoint protein 1 Nuclear factor with BRCT domains 1 MDC1 KIAA0170 NFBD1 |
Images
|
Fig1:
Western blot analysis of MDC1 on different lysates with Rabbit anti-MDC1 antibody (HA751892) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: HEK-293 (Human embryonic kidney cells) cell lysate Lane 3: K-562 (Human chronic myelogenous leukemia cells) cell lysate Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751892, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 227 kDa Observed band size: 227/260-320 kDa |
|
Fig2:
Western blot analysis of MDC1 on different lysates with Rabbit anti-MDC1 antibody (HA751892) at 1/5,000 dilution.
Lane 1: Rat testis tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Low expression : Rat liver (PMID: 32866776) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751892, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 227 kDa Observed band size: 227/260-320 kDa |
|
Fig3:
Western blot analysis of MDC1 on different lysates with Rabbit anti-MDC1 antibody (HA751892) at 1/5,000 dilution. Lane 1: HeLa -si WT cell lysate Lane 2: HeLa -si MDC1 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751892, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 227 kDa Observed band size: 227/260-320 kDa |
|
Fig4:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: NIH/3T3 (Mouse embryonic fibroblast) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA751892, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.