Thioredoxin Recombinant Rabbit Monoclonal Antibody [PSH23-52]
cat.: HA751900
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH23-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Thioredoxin aa 1-105. |
| Positive control: | HeLa (Human cervical adenocarcinoma cells) cell lysate, Hep G2 (Human liver cancer cells) cell lysate, MCF7 (Human breast cancer cells) cell lysate, A549 (Human lung adenocarcinoma cells) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, Neuro-2a (Mouse brain neuroblastoma cells) cell lysate, C2C12 (Mouse myoblasts) cell lysate, C6 (Rat glioma cells) cell lysate, PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: P10599 Human | P10639 Mouse | P11232 Rat |
| Alternative names: | ADF ATL derived factor ATL-derived factor DKFZp686B1993 MGC61975 SASP Surface associated sulphydryl protein Surface-associated sulphydryl protein testicular tissue protein Li 199 THIO_HUMAN Thioredoxin thioredoxin delta 3 TRDX TRX 1 Trx TRX1 TXN TXN delta 3 TXN protein zgc:92903 |
Images
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Fig1:
Western blot analysis of Thioredoxin on different lysates with Rabbit anti-Thioredoxin antibody (HA751900) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: Hep G2 (Human liver cancer cells) cell lysate Lane 3: MCF7 (Human breast cancer cells) cell lysate Lane 4: A549 (Human lung adenocarcinoma cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 9 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751900, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 12 kDa Observed band size: 12 kDa |
|
Fig2:
Western blot analysis of Thioredoxin on different lysates with Rabbit anti-Thioredoxin antibody (HA751900) at 1/5,000 dilution.
Lane 1: COS-1 (African green monkey kidney fibroblasts) cell lysate Lane 2: NIH/3T3 (Mouse fibroblasts) cell lysate Lane 3: Neuro-2a (Mouse brain neuroblastoma cells) cell lysate Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate Lane 5: C2C12 (Mouse myoblasts) cell lysate Lane 6: C6 (Rat glioma cells) cell lysate Lane 7: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate Lane 8: Mouse kidney tissue lysate Lane 9: Rat kidney tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 9 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751900, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 12 kDa Observed band size: 12 kDa |
|
Fig3:
Immunoprecipitation (IP)
Thioredoxin was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA751900 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751900 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751900 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751900 in HeLa cell lysate Exposure time: 2 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA751900, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 12 kDa Observed band size: 12 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.