MSR1 / CD204 Recombinant Rabbit Monoclonal Antibody [PSH23-60]
cat.: HA751902
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH23-60 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse MRS1 aa 79-458. |
| Positive control: | J774A.1 (Mouse monocyte macrophages) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate, RAW264.7 treated with 50nM PMA and 5ug/mL Lipopolysaccharide for 24 hours cell lysate, mouse liver tissue lysates. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P30204 Mouse |
| Alternative names: | CD204 CD204 antigen Macrophage acetylated LDL receptor I and II Macrophage scavenger receptor 1 Macrophage scavenger receptor type III Macrophage scavenger receptor types I and II Msr 1 MSR1 phSR1 phSR2 SCARA 1 SCARA1 Scavenger receptor class A member 1 Scavenger receptor class A, member 1 Scavenger receptor type A Scvr SR A SRA |
Images
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Fig1:
Western blot analysis of MSR1 / CD204 on different lysates with Rabbit anti-MSR1 / CD204 antibody (HA751902) at 1/5,000 dilution.
Lane 1: J774A.1 (Mouse monocyte macrophages) cell lysate Lane 2: Neuro-2a (Mouse brain neuroblastoma cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute; ECL: K1801 Neuro-2a is a negative control (PMID: 35937296). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751902, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 70-90 kDa |
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Fig2:
Western blot analysis of MSR1 / CD204 on different lysates with Rabbit anti-MSR1 / CD204 antibody (HA751902) at 1/5,000 dilution.
Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate Lane 2: RAW264.7 treated with 50nM PMA and 5ug/mL Lipopolysaccharide for 24 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 9 seconds; ECL: K1801 Blocking:5% NFDM/TBST , 1 hour at room temperature Primary antibody: HA751902, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 70-90 kDa |
|
Fig3:
Western blot analysis of MSR1 / CD204 on mouse liver tissue lysate with Rabbit anti-MSR1 / CD204 antibody (HA751902) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751902, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 70-90 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.