ABCC4 Recombinant Rabbit Monoclonal Antibody [PSH23-61]
cat.: HA751903
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: PSH23-61
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 150 kDa
Isotype: IgG
Immunogen: Recombinant protein within human ABCC4 aa 391-719.
Positive control: U-2 OS (Human osteosarcoma cells) cell lysate, SW620 (Human colon cancer cells) cell lysate, LNCaP (Human prostate cancer cells) cell lysate, HT-29 (Human colorectal adenocarcinoma cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, A549 (Human lung adenocarcinoma cells) cell lysate, NIH:OVCAR-3 (Human ovarian adenocarcinoma cells) cell lysate, Hep G2 (Human liver cancer cells) cell lysate, PC-12 (Rat pheochromocytoma cells (undifferentiated) cell lysate.
Subcellular location: Basolateral cell membrane, Apical cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
  IP
1:5,000
1:100
1:1,000
1:1,000
1-2μg/sample
Uniprot #: SwissProt: O15439 Human
Alternative names: ABCC 4 ABCC4 ATP binding cassette sub family C (CFTR/MRP) member 4 ATP binding cassette sub family C member 4 ATP-binding cassette sub-family C member 4 bA464I2.1 (ATP binding cassette sub-family C (CFTR/MRP) member 4) bA464I2.1 Canalicular multispecific organic anion transporter Canalicular multispecific organic anion transporter ABC superfamily EST170205 MOAT B MOAT-B MOATB MRP 4 MRP/cMOAT related ABC transporter MRP/cMOAT-related ABC transporter MRP4_HUMAN Multi specific organic anion transporter B Multi-specific organic anion transporter B Multidrug resistance associated protein 4 Multidrug resistance-associated protein 4 OTTHUMP00000018560
Images
HA751903_1.jpg Fig1: Western blot analysis of ABCC4 on different lysates with Rabbit anti-ABCC4 antibody (HA751903) at 1/5,000 dilution.

Lane 1: U-2 OS (Human osteosarcoma cells) cell lysate
Lane 2: SW620 (Human colon cancer cells) cell lysate
Lane 3: LNCaP (Human prostate cancer cells) cell lysate
Lane 4: HT-29 (Human colorectal adenocarcinoma cells) cell lysate
Lane 5: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 6: A549 (Human lung adenocarcinoma cells) cell lysate
Lane 7: NIH:OVCAR-3 (Human ovarian adenocarcinoma cells) cell lysate
Lane 8: Hep G2 (Human liver cancer cells) cell lysate
Lane 9: PC-12 (Rat pheochromocytoma cells (undifferentiated) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 30 seconds; ECL: K1801

Low expression of ABCC4 protein in U-2 OS cell is consistent with the predicted expression pattern

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751903, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 150 kDa
Observed band size: 140~200 kDa
HA751903_2.jpg Fig2: Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: LNCaP (Human Lymph Node Carcinoma of the Prostate Cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA751903, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.

Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
HA751903_3.jpg Fig3: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751903, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA751903_4.jpg Fig4: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Prostate
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751903, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA751903_5.jpg Fig5: Application: Flow Cytometry (Intra)

Species: Human
Sample: LNCaP (Human prostate cancer cells)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA751903 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
HA751903_6.jpg Fig6: Immunoprecipitation (IP)

ABCC4 was immunoprecipitated in 0.2 mg LNCaP (Human prostate cancer cells) cell lysate with HA751903 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751903 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: LNCaP cell lysate (input)
Lane 2: HA751903 IP in LNCaP cell lysate
Lane 3: Rabbit IgG instead of HA751903 in LNCaP cell lysate

Exposure time: 11 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature

Primary dilution: HA751903, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

Predicted band size: 150 kDa
Observed band size: 140~200 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.