MUC5B Recombinant Rabbit Monoclonal Antibody [PSH23-64]
cat.: HA751905
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH23-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 596 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MUC5B aa 1,201-1,550. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:1,000-1:5,000 1:2,000 |
| Uniprot #: | SwissProt: Q9HC84 Human |
| Alternative names: | 2300002I04Rik A130042M24 AV085033 Cervical mucin Cervical mucin MUC5B High molecular weight salivary mucin MG1 MG1 MUC-5B MUC5 MUC5B MUC5B_HUMAN MUC9 Mucin 5 subtype B, tracheobronchial Mucin 5B Mucin 5B oligomeric mucus/gel forming Mucin-5 subtype B Mucin-5B Sublingual gland mucin tracheobronchial |
Images
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Fig1:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Seromucous gland Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751905, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Gallbladder Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751905, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Pancreatic mucinous adenocarcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751905, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Gastric signet ring cell carcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751905, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of MUC5B protein in gastric signet ring cell carcinoma is consistent with the predicted expression pattern. The areas that stain positive are fundic glands with mucus differentiation adjacent to human gastric signet ring cell carcinoma. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Pancreatic mucinous adenocarcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751905, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Stomach Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751905, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. The unstained gastric surface cells were consistent with the predicted expression pattern. |
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Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Cervix (endocervix) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751905, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig8:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Gastric signet ring cell carcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751905, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of MUC5B protein in gastric signet ring cell carcinoma is consistent with the predicted expression pattern. |
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Fig9:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Cervix (ectocervix) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751905, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of MUC5B protein in ectocervix is consistent with the predicted expression pattern. |
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Fig10:
Application: Immunofluorescence (IF-tissue)
Species: Human Tissue: Seromucous gland Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751905, 1/2,000, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.