COX5A Recombinant Rabbit Monoclonal Antibody [PSH23-69]
cat.: HA751908
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH23-69 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human COX5A aa 1-150. |
| Positive control: | HeLa (Human cervical adenocarcinoma cells) cell lysate, Hep G2 (Human liver cancer cells) cell lysate, A431 (Human epidermoid carcinoma skin squamous cells) cell lysate, HEK-293 (Human embryonic kidney cells) cell lysate, Mouse heart tissue lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat heart tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P20674 Human | P12787 Mouse | P11240 Rat |
| Alternative names: | COX COX VA COX5A COX5A_HUMAN Cytochrome c oxidase polypeptide Va Cytochrome c oxidase polypeptide, mitochondrial Cytochrome c oxidase subunit 5A Cytochrome c oxidase subunit 5A, mitochondrial Cytochrome c oxidase subunit Va mitochondrial Mitochondrial cytochrome c oxidase subunit Va VA |
Images
|
Fig1:
Western blot analysis of COX5A on different lysates with Rabbit anti-COX5A antibody (HA751908) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: Hep G2 (Human liver cancer cells) cell lysate Lane 3: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate Lane 4: HEK-293 (Human embryonic kidney cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751908, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 17 kDa Observed band size: 13 kDa |
|
Fig2:
Western blot analysis of COX5A on different lysates with Rabbit anti-COX5A antibody (HA751908) at 1/5,000 dilution.
Lane 1: Mouse heart tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse kidney tissue lysate Lane 5: Rat heart tissue lysate Lane 6: Rat liver tissue lysate Lane 7: Rat brain tissue lysate Lane 8: Rat kidney tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751908, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 17 kDa Observed band size: 13 kDa |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751908, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751908, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.