Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH23-74]
cat.: HA751909
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH23-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 117 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Eph receptor B2 aa 1-543. |
| Positive control: | COLO205 (Human colon adenocarcinoma cells) cell lysate, K-562 (Human chronic myelogenous leukemia cells) cell lysate, Saos-2 (Human osteosarcoma cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, 4T1 (Mouse breast cancer cells) cell lysate, Mouse brain tissue lysate, Mouse colon tissue lysate, Rat brain tissue lysate, Rat colon tissue lysate. |
| Subcellular location: | Cell membrane, Cell projection, axon, dendrite. |
| Recommended Dilutions:
WB IHC-Fr IP |
1:5,000 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: P29323 Human | P54763 Mouse Entrez Gene: 313633 Rat |
| Alternative names: | cek5 Developmentally regulated EPH related tyrosine kinase DRT EK5 ELK related protein tyrosine kinase Eph receptor B2 EPH tyrosine kinase 3 EPH-like kinase 5 EPHB2 EPHB2_HUMAN Ephrin type B receptor 2 Ephrin type-B receptor 2 EPHT 3 ERK ETECK hEK5 Nuk Prkm 5 Receptor protein tyrosine kinase HEK 5 Renal carcinoma antigen NY-REN-47 Sek 3 Tyro 5 Tyrosine protein kinase receptor CEK 5 Tyrosine protein kinase receptor EPH 3 Tyrosine protein kinase receptor QEK 5 Tyrosine-protein kinase receptor EPH-3 Tyrosine-protein kinase TYRO5 |
Images
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Fig1:
Western blot analysis of Eph receptor B2 on different lysates with Rabbit anti-Eph receptor B2 antibody (HA751909) at 1/5,000 dilution.
Lane 1: COLO205 (Human colon adenocarcinoma cells) cell lysate Lane 2: K-562 (Human chronic myelogenous leukemia cells) cell lysate Lane 3: Saos-2 (Human osteosarcoma cells) cell lysate Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate Lane 5: 4T1 (Mouse breast cancer cells) cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Mouse colon tissue lysate Lane 8: Rat brain tissue lysate Lane 9: Rat colon tissue lysate Cell lysates/proteins at 20 µg/Lane. Tissue lysates/proteins at 40 µg/Lane. Exposure time: 3 minutes; ECL: K1801 K-562 is a negative control (PMID : 18713744, PMID : 21508957). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751909, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 117 kDa Observed band size: 130 kDa |
|
Fig2:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Colon Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA751909, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig3:
Application: Immunofluorescence (IHC-Fr)
Species: Rat Tissue: Colon Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA751909, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig4:
Immunoprecipitation (IP)
Eph receptor B2 was immunoprecipitated in 0.2 mg COLO205 (Human colon adenocarcinoma cells) cell lysate with HA751909 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751909 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: COLO205 cell lysate (input) Lane 2: HA751909 IP in COLO205 cell lysate Lane 3: Rabbit IgG instead of HA751909 in COLO205 cell lysate Exposure time: 2 minutes 6 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA751909, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 117 kDa Observed band size: 130 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.