Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH23-93]
cat.: HA751919
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH23-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 166 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Mannose Receptor(CD206) aa 1-1,389. |
| Positive control: | THP-1 treated with 20ng/ml PMA for 24 hours then treated with 20ng/ml IL-4 for 48 hours cell lysate. |
| Subcellular location: | Endosome membrane, Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IP |
1:5,000 1:1,000-1:3,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P22897 Human |
| Alternative names: | bA541I19.1 C type lectin domain family 13 member D C-type lectin domain family 13 member D CD 206 CD206 CD206 antigen CLEC13D CLEC13DL Macrophage mannose receptor 1 Macrophage mannose receptor 1 like protein 1 Macrophage mannose receptor Mannose receptor C type 1 Mannose receptor C type 1 like 1 MMR MRC 1 MRC1 MRC1_HUMAN MRC1L1 OTTHUMP00000045206 |
Images
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Fig1:
Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (HA751919) at 1/5,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cells) cell lysate Lane 2: THP-1 treated with 20ng/ml PMA for 24 hours then treated with 20ng/ml IL-4 for 48 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751919, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 166 kDa Observed band size: 180 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751919, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751919, 1/3,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751919, 1/3,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunofluorescence (IF-tissue) Species: Human Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751919, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig6:
Immunoprecipitation (IP) Mannose Receptor(CD206) was immunoprecipitated in 0.2 mg THP-1 (Human acute monoblastic leukemia cell) treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate with HA751919 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751919 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate Lane 2: HA751919 IP in THP-1 treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate Lane 3: Rabbit IgG instead of HA751919 in THP-1 treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate Exposure time: 3 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA751919, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 166 kDa Observed band size: 250 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.