Involucrin Recombinant Rabbit Monoclonal Antibody [PSH23-99]
cat.: HA751921
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH23-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Involucrin aa 1-585. |
| Positive control: | HaCaT (Human immortalized keratinocytes) cell lysate, A431 (Human epidermoid carcinoma skin squamous cells) cell lysate, SK-BR-3 (Human breast cancer cells) cell lysate, NCI-H441 (Human lung adenocarcinoma cells) cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:4,000 |
| Uniprot #: | SwissProt: P07476 Human |
| Alternative names: | INVO_HUMAN Involucrin IVL |
Images
|
Fig1:
Western blot analysis of Involucrin on different lysates with Rabbit anti-Involucrin antibody (HA751921) at 1/5,000 dilution. Lane 1: HaCaT (Human immortalized keratinocytes) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 3: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate Lane 4: SK-BR-3 (Human breast cancer cells) cell lysate Lane 5: NCI-H441 (Human lung adenocarcinoma cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 7 secondss; ECL: K1801 Negative expression of Involucrin protein in HeLa cell is consistent with the predicted expression pattern Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751921, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 68 kDa Observed band size: 100~150 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751921, 1/4,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751921, 1/4,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.