Endomucin Recombinant Rabbit Monoclonal Antibody [PSH24-00]
cat.: HA751922
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH24-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 0.2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Endomucin aa 1-190. |
| Positive control: | Mouse kidney tissue lysate, Mouse thyroid tissue lysate, Mouse lung tissue lysate, Mouse heart tissue lysate, Mouse ovary tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:100,000 1:400 |
| Uniprot #: | SwissProt: Q9R0H2 Mouse |
| Alternative names: | EMCN EMCN2 Endomucin 2 Endomucin Endomucin-2 Endothelial sialomucin Gastric cancer antigen Ga34 MUC-14 MUC14 MUCEN_HUMAN Mucin 14 Mucin-14 |
Images
|
Fig1:
Western blot analysis of Endomucin on different lysates with Rabbit anti-Endomucin antibody (HA751922) at 1/100,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Mouse thyroid tissue lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse heart tissue lysate Lane 6: Mouse ovary tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Low expression : Mouse brain (PMID: 12000719). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751922, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28 kDa Observed band size: 70~100 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751922, 1/400, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.