NR4A3 Recombinant Rabbit Monoclonal Antibody [PSH24-01]
cat.: HA751923
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH24-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse NR4A3 aa 185-234. |
| Positive control: | THP-1 (Human acute monoblastic leukemia cells) cell lysate, THP-1 treated with 20ng/ml TPA for 24 hours and 20ng/ml IL-4 for 48 hours cell lysate, THP-1 treated with 50ng/ml TPA for 72 hours and 1μg/ml LPS for 24 hours cell lysate, THP-1 treated with 80nM TPA for 16 hours and 20ng/ml hIFN-γ for 8 hours cell lysate, MDA-MB-231 (Human breast cancer cells) cell lysate, MCF7 (Human breast cancer cells) cell lysate, U-2 OS (Human osteosarcoma cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q92570 Human |
| Alternative names: | CHN Chondrosarcoma extraskeletal myxoid fused to EWS CSMF MINOR Mitogen induced nuclear orphan receptor Mitogen-induced nuclear orphan receptor Neuron derived orphan receptor 1 Neuron derived orphan receptor Neuron-derived orphan receptor 1 NOR1 Nr4a3 NR4A3_HUMAN Nuclear hormone receptor NOR-1 Nuclear hormone receptor NOR1 Nuclear receptor subfamily 4 group A member 3 TEC Translocated in extraskeletal chondrosarcoma |
Images
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Fig1:
Western blot analysis of NR4A3 on different lysates with Rabbit anti-NR4A3 antibody (HA751923) at 1/5,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cells) cell lysate Lane 2: THP-1 treated with 20ng/ml TPA for 24 hours and 20ng/ml IL-4 for 48 hours cell lysate Lane 3: THP-1 (Human acute monoblastic leukemia cells) cell lysate Lane 4: THP-1 treated with 50ng/ml TPA for 72 hours and 1μg/ml LPS for 24 hours cell lysate Lane 5: THP-1 (Human acute monoblastic leukemia cells) cell lysate Lane 6: THP-1 treated with 80nM TPA for 16 hours and 20ng/ml hIFN-γ for 8 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751923, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 68 kDa Observed band size: 68 kDa |
|
Fig2:
Western blot analysis of NR4A3 on different lysates with Rabbit anti-NR4A3 antibody (HA751923) at 1/5,000 dilution.
Lane 1: MDA-MB-231 (Human breast cancer cells) cell lysate Lane 2: MCF7 (Human breast cancer cells) cell lysate Lane 3: U-2 OS (Human osteosarcoma cells) cell lysate Lane 4: HeLa (Human cervical adenocarcinoma cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751923, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 68 kDa Observed band size: 68 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.