Phospho-TAK1 (T184, T187) Recombinant Rabbit Monoclonal Antibody [PSH24-03]
cat.: HA751924
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH24-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr184 and Thr187 of Human TAK1. |
| Positive control: | HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: O43318 Human | Q62073 Mouse |
| Alternative names: | M3K7_HUMAN MAP3K 7 Map3k7 MEKK7 Mitogen activated protein kinase kinase kinase 7 Mitogen-activated protein kinase kinase kinase 7 TAK1 TGF beta activated kinase 1 TGF-beta-activated kinase 1 TGF1a Transforming growth factor beta activated kinase 1 Transforming growth factor-beta-activated kinase 1 |
Images
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Fig1:
Western blot analysis of Phospho-TAK1 (T184, T187) on different lysates with Rabbit anti-Phospho-TAK1 (T184, T187) antibody (HA751924) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cells) Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: NIH/3T3 (Mouse fibroblasts) Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Total TAK1 protein was detected with another antibody ( Huabio, ET1705-14) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751924, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 67 kDa Observed band size: 67 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.