OASL Recombinant Rabbit Monoclonal Antibody [PSH24-15]
cat.: HA751929
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH24-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human OASL aa 315-514. |
| Positive control: | BxPC-3 (Human primary pancreatic adenocarcinoma cells) cell lysate, U-87 MG (Human glioblastoma cells) cell lysate, THP-1 (Human acute monoblastic leukemia cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, A549 treated with 100ng/ml hIFN-α2 for 24 hours cell lysate. |
| Subcellular location: | Nucleus, nucleolus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:5,000 1:1,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q15646 Human |
| Alternative names: | 2' 5' oligoadenylate synthetase like 2''-5''-OAS-related protein 2''-5''-OAS-RP 2''-5''-oligoadenylate synthase-like protein 2'-5' oligoadenylate synthetase-like 2 2'-5'-oligoadenylate synthetase-like 59 kDa 2' 5' oligoadenylate synthetase like protein 59 kDa 2''-5''-oligoadenylate synthase-like protein 59 kDa 2'-5'-oligoadenylate synthetase-like protein M1204 Mmu-OASL OASL OASL_HUMAN Oasl2 p59 OASL p59OASL THYROID HORMONE RECEPTOR INTERACTOR 14 Thyroid receptor interacting protein 14 Thyroid receptor-interacting protein 14 TR-interacting protein 14 TRIP-14 TRIP14 |
Images
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Fig1:
Western blot analysis of OASL on different lysates with Rabbit anti-OASL antibody (HA751929) at 1/5,000 dilution.
Lane 1: BxPC-3 (Human primary pancreatic adenocarcinoma cells) cell lysate Lane 2: U-87 MG (Human glioblastoma cells) cell lysate Lane 3: HepG2 (Human liver cancer cells) cell lysate Lane 4: THP-1 (Human acute monoblastic leukemia cells) cell lysate Lane 5: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 6: A549 (Human lung adenocarcinoma cells) cell lysate Lane 7: A549 treated with 100ng/ml hIFN-α2 for 24 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Negative expression of OASL protein in HepG2 cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751929, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 59 kDa Observed band size: 50 kDa |
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Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751929, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751929, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751929, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Breast Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751929, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of OASL protein in breast is consistent with the predicted expression pattern. |
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Fig6:
Application: Flow Cytometry (Intra)
Species: Human Sample: A549 (Human lung adenocarcinoma cells) treated with hIFN-α2(100ng/ml 24h) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA751929 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
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Fig7:
Immunoprecipitation (IP) OASL was immunoprecipitated in 0.2 mg U-87 MG (Human glioblastoma cells) cell lysate with HA751929 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751929 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: U-87 MG cell lysate (input) Lane 2: HA751929 IP in U-87 MG cell lysate Lane 3: Rabbit IgG instead of HA751929 in U-87 MG cell lysate Exposure time: 5 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA751929, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 59 kDa Observed band size: 50 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.