GC / Vitamin D Binding protein Recombinant Rabbit Monoclonal Antibody [PSH24-22]
cat.: HA751932
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH24-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Positive control: | Human plasma tissue lysate, Human serum tissue lysate, Mouse plasma tissue lysate, Rat plasma tissue lysate, HepG2 (Human liver cancer cells) cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Mouse eyebal tissue lysate, Rat eyeball tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P02774 Human | P21614 Mouse | P04276 Rat |
| Alternative names: | DBP DBP/GC GC Gc globulin Gc-globulin GRD3 Group specific component Group specific component vitamin D binding protein Group-specific component hDBP VDB VDBG VDBP Vitamin D binding alpha globulin Vitamin D-binding protein VTDB_HUMAN |
Images
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Fig1:
Western blot analysis of GC / Vitamin D Binding protein on different lysates with Rabbit anti-GC / Vitamin D Binding protein antibody (HA751932) at 1/5,000 dilution.
Lane 1: Human plasma tissue lysate Lane 2: Human serum tissue lysate Lane 3: Mouse plasma tissue lysate Lane 4: Rat plasma tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 34 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751932, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 53 kDa Observed band size: 53 kDa |
|
Fig2:
Western blot analysis of GC / Vitamin D Binding protein on different lysates with Rabbit anti-GC / Vitamin D Binding protein antibody (HA751932) at 1/5,000 dilution.
Lane 1: HepG2 (Human liver cancer cells) cell lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lane 4: Mouse eyebal tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 34 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751932, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 53 kDa Observed band size: 53 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.