DNAJC5 Recombinant Rabbit Monoclonal Antibody [PSH24-38]
cat.: HA751939
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey, Pig |
| Applications: | WB, IF-Cell, IHC-Fr, IHC-P, FC(Intra), IP |
| Clonality: | Monoclonal |
| Clone number: | PSH24-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DNAJC5 aa 151-198. |
| Positive control: | HeLa (Human cervical adenocarcinoma cells) cell lysate, THP-1 (Human acute monoblastic leukemia cells) cell lysate, HepG2 (Human liver cancer cells) cell lysate, Huh7 (Human liver cancer cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate, PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate, C6 (Rat glioma cells) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Membrane, Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane , Melanosome, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-Fr IHC-P FC(Intra) IP |
1:5,000 1:100 1:500 1:200-1:1,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9H3Z4 Human | P60904 Mouse | P60905 Rat |
| Alternative names: | CLN 4 CLN4 CLN4B CSP cysteine string protein alpha Cysteine string protein DJC5 DnaJ (Hsp40) homolog subfamily C member 5 DnaJ homolog subfamily C member 5 DNAJC 5 Dnajc5 DNAJC5A DNJC5_HUMAN NCL |
Images
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Fig1:
Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (HA751939) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: THP-1 (Human acute monoblastic leukemia cells) cell lysate Lane 3: HepG2 (Human liver cancer cells) cell lysate Lane 4: Huh7 (Human liver cancer cells) cell lysate Lane 5: NIH/3T3 (Mouse fibroblasts) cell lysate Lane 6: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate Lane 7: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate Lane 8: C6 (Rat glioma cells) cell lysate Lane 9: COS-1 (African green monkey kidney fibroblasts) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 7 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751939, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22 kDa Observed band size: 34 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Rat Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated)) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA751939, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
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Fig3:
Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (HA751939) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat kidney tissue lysate Lane 5: Pig brain tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 7 seconds; ECL: K1801 Negative expression of DNAJC5 protein in kidney tissue is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751939, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22 kDa Observed band size: 34 kDa |
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Fig4:
Application: Immunofluorescence (IHC-Fr)
Species: Rat Tissue: Brain Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751939, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig5:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Brain Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751939, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Placenta Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751939, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751939, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig8:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751939, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig9:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Placenta Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751939, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig10:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751939, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig11:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751939, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig12:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Placenta Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751939, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig13:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751939, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig14:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751939, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig15:
Application: Flow Cytometry (Intra)
Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA751939 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
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Fig16:
Application: Flow Cytometry (Intra)
Species: Mouse Sample: NIH/3T3 (Mouse fibroblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA751939 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
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Fig17:
Application: Flow Cytometry (Intra)
Species: Rat Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated)) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA751939 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
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Fig18:
Immunoprecipitation (IP) DNAJC5 was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA751939 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751939 at 1/25,000 dilution. Alpaca anti-Rabbit IgG Fc, Recombinant VHH monoclonal Antibody(HA1031) at 1/50000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751939 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751939 in HeLa cell lysate Exposure time: 2 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA751939, 1/25,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 22 kDa Observed band size: 34 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.