CACNA1C Recombinant Rabbit Monoclonal Antibody [PSH24-46]
cat.: HA751942
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH24-46 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 240 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CACNA1C aa 1,651-2,050. |
| Positive control: | Mouse Cardiac muscle, Mouse Colon, Rat Cardiac muscle, Rat Colon. |
| Subcellular location: | Cell membrane, sarcolemma, Perikaryon, Postsynaptic density membrane, Cell projection, dendrite, T-tubule. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:500 1:1,000 |
| Uniprot #: | SwissProt: Q01815 Mouse | P22002 Rat |
| Alternative names: | CACH 2 CACH2 CACN 2 CACN2 CACNA1C CACNL1A1 Calcium channel Calcium channel cardic dihydropyridine sensitive alpha 1 subunit Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle Calcium channel voltage dependent L type alpha 1C subunit CaV1.2 CCHL1A1 DHPR alpha 1 DHPR alpha 1 subunit LQT8 TS Voltage dependent L type calcium channel alpha 1C subunit Voltage dependent L type calcium channel subunit alpha 1C Voltage gated calcium channel alpha subunit Cav1.2 Voltage gated calcium channel subunit alpha Cav1.2 Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* Voltage-dependent L-type calcium channel subunit alpha-1C Voltage-gated calcium channel subunit alpha Cav1.2 |
Images
|
Fig1:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Cardiac muscle Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751942, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Cardiac muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751942, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751942, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Cardiac muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751942, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751942, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.