CSPG4 Recombinant Rabbit Monoclonal Antibody [PSH24-57]
cat.: HA751947
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH24-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 251 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CSPG4 aa 151-600. |
| Positive control: | A375 (Human amelanotic melanoma cells) cell lysate, SK-MEL-28 (Human malignant melanoma cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, Mouse brain tissue lysate, Mouse spinal cord tissue lysate, Rat brain tissue lysate, Rat pancreas tissue lysate. |
| Subcellular location: | Cell membrane, Apical cell membrane, Cell projection, lamellipodium membrane, Cell surface. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue FC |
1:5,000 1:200 1:1,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: Q6UVK1 Human | Q8VHY0 Mouse | Q00657 Rat |
| Alternative names: | 4732461B14Rik AN2 AN2 proteoglycan Chondroitin sulfate proteoglycan 4 (melanoma-associated) Chondroitin sulfate proteoglycan 4 Chondroitin sulfate proteoglycan NG2 Cspg4 Cspg4 chondroitin sulfate proteoglycan 4 CSPG4_HUMAN HMW-MAA HSN tumor-specific antigen Kiaa4232 MCSP MCSPG MEL-CSPG Melanoma chondroitin sulfate proteoglycan Melanoma-associated chondroitin sulfate proteoglycan MELCSPG MSK16 NG2 |
Images
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Fig1:
Western blot analysis of CSPG4 on different lysates with Rabbit anti-CSPG4 antibody (HA751947) at 1/5,000 dilution.
Lane 1: A375 (Human amelanotic melanoma cells) cell lysate Lane 2: SK-MEL-28 (Human malignant melanoma cells) cell lysate Lane 3: MCF7 (Human breast cancer cells) cell lysate Lane 4: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 5: SK-BR-3 (Human breast cancer cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 9 seconds; ECL: K1801 MCF7 is a negative control (PMID: 20852124). SK-BR-3 is a negative control (PMID: 20852124). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751947, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 251 kDa Observed band size: 280,330 kDa |
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Fig2:
Western blot analysis of CSPG4 on different lysates with Rabbit anti-CSPG4 antibody (HA751947) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Mouse spinal cord tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat liver tissue lysate Lane 6: Rat pancreas tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Liver is a negative control (PMID: 23481707). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751947, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 251 kDa Observed band size: 330 kDa |
|
Fig3:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: A375 (Human amelanotic melanoma cells)/SK-BR-3 (Human breast cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA751947, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). Negative control: SK-Br-3 (PMID: 20852124). |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Choroid plexus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751947, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751947, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751947, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Mouse liver tissue is a negative control (PMID: 23481707). |
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Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751947, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig8:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA751947, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Rat liver tissue is a negative control (PMID: 23481707). |
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Fig9:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751947, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig10:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Liver is a negative control (PMID: 23481707). Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751947, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig11:
Application: Immunofluorescence (IF-tissue)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751947, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig12:
Application: Immunofluorescence (IF-tissue)
Species: Rat Tissue: Liver Sample: Paraffin-embedded section Liver is a negative control (PMID: 23481707). Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751947, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig13:
Application: Flow Cytometry
Species: Human Sample: A375 (Human amelanotic melanoma cells)/SK-BR-3 (Human breast cancer cells) Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA751947 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative control: SK-Br-3 (PMID: 20852124). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.