CCL5 Recombinant Rabbit Monoclonal Antibody [PSH24-74]
cat.: HA751952
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 10 kDa |
| Isotype: | IgG |
| Positive control: | NK-92 (Human natural killer cells) cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell FC(Intra) |
1:5,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: P13501 Human |
| Alternative names: | Beta chemokine RANTES Beta chemokine RANTES precursor C C motif chemokine 5 CCL 5 CCL5 CCL5_HUMAN Chemokine (C C motif) ligand 5 Chemokine CC Motif Ligand 5 D17S136E EoCP Eosinophil chemotactic cytokine MGC17164 RANTES(4-68) Regulated upon activation normally T expressed and presumably secreted SCYA 5 SCYA5 SIS delta SIS-delta SISd Small inducible cytokine A5 (RANTES) Small inducible cytokine A5 Small inducible cytokine subfamily A (Cys Cys) member 5 Small-inducible cytokine A5 T cell specific protein p288 T cell specific protein RANTES T cell specific RANTES protein T cell-specific protein P228 T-cell-specific protein RANTES TCP 228 TCP228 |
Images
|
Fig1:
Western blot analysis of CCL5 on different lysates with Rabbit anti-CCL5 antibody (HA751952) at 1/5,000 dilution.
Lane 1: NK-92 (Human natural killer cells) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; ECL: K1802 Jurkat is a negative control (PMID: 15239133). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751952, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 10 kDa Observed band size: 10 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: NK-92 (Human natural killer cells)/Jurkat (Human T-lymphoblastic cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA751952, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). Negative control: Jurkat (PMID:15239133). |
|
Fig3:
Application: Flow Cytometry (Intra)
Species: Human Sample: NK-92 (Human natural killer cells)/Jurkat (Human T-lymphoblastic cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA751952 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative control: Jurkat (PMID: 15239133). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.