iNOS Recombinant Rabbit Monoclonal Antibody [PSH24-75]
cat.: HA751953
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH24-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 130 kDa |
| Isotype: | IgG |
| Positive control: | Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, RAW264.7 treated with 100ng/mL LPS for 24h cell lysate, NR8383 (Rat alveolar macrophage) cell lysate, NR8383 treated with 200ng/mL LPS for 4h add 1μg/mL BFA for last 2h cell lysate. |
| Subcellular location: | Cytoskeleton, Nucleus, Cytosol. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:1,000 1:10,000 |
| Uniprot #: | SwissProt: P35228 Human | P29477 Mouse | Q06518 Rat |
| Alternative names: | HEP-NOS Hepatocyte NOS HEPNOS inducible Inducible nitric oxide synthase Inducible NO synthase Inducible NOS iNOS MAC NOS Macrophage NOS Nitric oxide synthase 2 inducible Nitric oxide synthase 2 inducible macrophage nitric oxide synthase 2A (inducible, hepatocytes) Nitric oxide synthase Nitric oxide synthase inducible nitric oxide synthase, macrophage NOS 2 NOS Nos II NOS type II nos2 NOS2_HUMAN NOS2A NOS2A, Inducible, Hepatocyte Peptidyl-cysteine S-nitrosylase NOS2 |
Images
|
Fig1:
Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (HA751953) at 1/5,000 dilution.
Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate Lane 2: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 3: RAW264.7 treated with 100ng/mL LPS for 24h cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751953, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 130 kDa Observed band size: 130 kDa |
|
Fig2:
Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (HA751953) at 1/5,000 dilution.
Lane 1: NR8383 (Rat alveolar macrophage) cell lysate Lane 2: NR8383 treated with 200ng/mL LPS for 4h add 1μg/mL BFA for last 2h cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751953, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 130 kDa Observed band size: 130 kDa |
|
Fig3:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell) treated with 100ng/mL LPS for 24h Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA751953, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig4:
Application: Flow Cytometry (Intra)
Species: Mouse Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell) treated with 100ng/mL LPS for 24h Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA751953(1/10,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.