ICAM2 Recombinant Rabbit Monoclonal Antibody [PSH25-72]
cat.: HA751991
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH25-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ICAM2 aa 1-248. |
| Positive control: | Jurkat (Human T-lymphoblastic cells) cell lysate, EA.hy926 (Human umbilical vein fusion cell) cell lysate. |
| Subcellular location: | Cell membrane, Cell projection, microvillus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P13598 Human | P35330 Mouse |
| Alternative names: | CD102 CD102 antigen ICAM 2 ICAM-2 Icam2 ICAM2_HUMAN Intercellular adhesion molecule 2 Ly60 |
Images
|
Fig1:
Western blot analysis of ICAM2 on different lysates with Rabbit anti-ICAM2 antibody (HA751991) at 1/5,000 dilution.
Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: HEK-293 (Human embryonic kidney cell) cell lysate Lane 4: EA.hy926 (Human umbilical vein fusion cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 HeLa is a negative control (PMID: 23714211). HEK-293 is a negative control (PMID: 23714211). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751991, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 31 kDa Observed band size: 55 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: Jurkat (Human T-lymphoblastic cells)/HEK-293 (Human embryonic kidney cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA751991, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Negative control: HEK-293 (PMID:23714211). |
|
Fig3:
Application: Flow Cytometry Species: Human Sample: HEK-293 (Human embryonic kidney cell)/Jurkat (Human T-lymphoblastic cells) Blocking: 1% BSA + 10% normal goat serum + human FcR (Miltenyi Biotec), 15 minutes at room temperature. (human) Antibody dilution buffer: 1x PBS. Primary antibody: HA751991 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative control: HEK-293 (PMID: 23714211). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.