FGFR2 Recombinant Rabbit Monoclonal Antibody [PSH26-90]
cat.: HA752052
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH26-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FGFR2 aa 1-400. |
| Positive control: | Saos-2 (Human osteosarcoma cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, HCT 116 (Human colon cancer cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate. |
| Subcellular location: | Cell membrane, Golgi apparatus, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB FC IP |
1:1,000-1:5,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P21802 Human | P21803 Mouse |
| Alternative names: | bacteria-expressed kinase BBDS BEK BEK fibroblast growth factor receptor BFR1 CD332 CD332 antigen CEK3 CFD1 Craniofacial dysostosis 1 ECT1 FGF receptor FGFR 2 FGFR-2 Fgfr2 FGFR2_HUMAN Fibroblast growth factor receptor 2 Hydroxyaryl protein kinase Jackson Weiss syndrome JWS K SAM K-sam Keratinocyte growth factor receptor 2 Keratinocyte growth factor receptor KGFR KSAM protein tyrosine kinase, receptor like 14 soluble FGFR4 variant 4 TK14 TK25 |
Images
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Fig1:
Western blot analysis of FGFR2 on different lysates with Rabbit anti-FGFR2 antibody (HA752052) at 1/1,000 dilution.
Lane 1: Saos-2 (Human osteosarcoma cell) cell lysate Lane 2: SW620 (Human colon cancer cell) cell lysate Lane 3: HT-29 (Human colorectal adenocarcinoma cell) cell lysate Lane 4: HCT 116 (Human colon cancer cell) cell lysate Lane 5: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 2 seconds; ECL: K1801 FGFR2/KGFR/K-sam recognizes endogenous levels of total FGFR2 protein and a C-terminal truncated form K-sam (PMID: 17505008). Negative control: SW620 (PMID: 24968263) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA752052, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 92 kDa Observed band size: 145 kDa |
|
Fig2:
Western blot analysis of FGFR2 on different lysates with Rabbit anti-FGFR2 antibody (HA752052) at 1/5,000 dilution.
Lane 1: Saos-2 (Human osteosarcoma cell) cell lysate Lane 2: Saos-2 treated with Deglycosylation cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute ; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA752052, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 92 kDa Observed band size: 145 kDa |
|
Fig3:
Application: Flow Cytometry Species: Human Sample: SW620 (Human colon cancer cell) (Left, Negative) / Saos-2 (Human osteosarcoma cell) (Right) Blocking: 1% BSA + 10% normal goat serum + human FcR (Miltenyi Biotec), 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA752052 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative control: SW620 (PMID: 24968263). |
|
Fig4:
Immunoprecipitation (IP) FGFR2 was immunoprecipitated in 0.2 mg Saos-2 (Human osteosarcoma cell) cell lysate with HA752052 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA752052 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Saos-2 cell lysate (input) Lane 2: HA752052 IP in Saos-2 cell lysate Lane 3: Rabbit IgG instead of HA752052 in Saos-2 cell lysate Exposure time: 3 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA752052, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 92 kDa Observed band size: 145 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.