PIWIL2 Recombinant Rabbit Monoclonal Antibody [PSH27-16]
cat.: HA752069
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB, IHC-Fr, IHC-P, IP
Clonality: Monoclonal
Clone number: PSH27-16
Form: Liquid
Storage condition: Store at 2-8℃. Avoid freeze.
Storage buffer: PBS (pH7.4).
Concentration: 0.5ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 109 kDa
Isotype: IgG
Immunogen: Recombinant protein within mouse PIWIL2 aa 1-250.
Positive control: Rat testis tissue lysate, Mouse testis tissue lysate.
Subcellular location: Cytoplasm.
Uniprot #: SwissProt: Q8CDG1 Mouse
Entrez Gene: 306011 Rat
Alternative names: Piwi-like protein 2 EC:3.1.26. Piwil2 Mili
Images
HA752069_1.jpg Fig1: This data was developed using HA752069, the same antibody clone in a different buffer formulation.
Western blot analysis of PIWIL2 on different lysates with Rabbit anti-PIWIL2 antibody (HA752069) at 1/2,000 dilution.

Lane 1: Rat testis tissue lysate
Lane 2: Rat brain tissue lysate

Negative control: Brain (PMID: 16377660).

Lysates/proteins at 30 µg/Lane.
Exposure time: 20 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752069, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 109 kDa
Observed band size: 109 kDa
HA752069_2.jpg Fig2: This data was developed using HA752069, the same antibody clone in a different buffer formulation.
Western blot analysis of PIWIL2 on different lysates with Rabbit anti-PIWIL2 antibody (HA752069) at 1/2,000 dilution.

Lane 1: Mouse testis tissue lysate
Lane 2: Mouse brain tissue lysate

Negative control: Brain (PMID: 16377660).

Lysates/proteins at 30 µg/Lane.
Exposure time: 20 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752069, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 109 kDa
Observed band size: 109 kDa
HA752069_3.jpg Fig3: This data was developed using HA752069, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IHC-Fr)

Species: Mouse
Tissue: Testis
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

Primary antibody: HA752069, 1/250, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
HA752069_4.jpg Fig4: This data was developed using HA752069, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Testis
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752069, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA752069_5.jpg Fig5: This data was developed using HA752069, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752069, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.

Negative expression of PIWIL2 protein in brain is consistent with the predicted expression pattern.
HA752069_6.jpg Fig6: This data was developed using HA752069, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Testis
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752069, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA752069_7.jpg Fig7: This data was developed using HA752069, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752069, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.

Negative expression of PIWIL2 protein in brain is consistent with the predicted expression pattern.
HA752069_8.jpg Fig8: This data was developed using HA752069, the same antibody clone in a different buffer formulation.
Immunoprecipitation (IP)

PIWIL2 was immunoprecipitated in 0.2 mg mouse testis tissue lysate with HA752069 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA752069 at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: Mouse testis tissue lysate (input)
Lane 2: HA752069 IP in mouse testis tissue lysate
Lane 3: Rabbit IgG instead of HA752069 in mouse testis tissue lysate

Exposure time: 1 minute 50 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature

Primary dilution: HA752069, 1/2,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

Predicted band size: 109 kDa
Observed band size: 109 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.