SERPING1 Recombinant Rabbit Monoclonal Antibody [PSH28-15]
cat.: HA752101
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: PSH28-15
Form: Liquid
Storage condition: Store at 2-8℃. Avoid freeze.
Storage buffer: PBS (pH7.4).
Concentration: 0.5ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG
Positive control: Hep G2 (Human hepatocellular carcinoma cell) cell lysates, Human serum lysate, Human urine lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Mouse plasma lysate, Rat plasma lysate.
Subcellular location: Secreted.
Uniprot #: SwissProt: P05155 Human | P97290 Mouse | Q6P734 Rat
Alternative names: C1 esterase inhibitor C1 Inh C1 inhibiting factor C1 inhibitor C1-inhibiting factor C1IN C1Inh C1NH complement component 1 inhibitor esterase inhibitor HAE1 HAE2 IC1_HUMAN Plasma protease C1 inhibitor Serine (or cysteine) proteinase inhibitor clade G member 1 serine/cysteine proteinase inhibitor clade G member 1 Serpin G1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 SERPING1
Images
HA752101_1.jpg Fig1: This data was developed using HA752101, the same antibody clone in a different buffer formulation.
Western blot analysis of SERPING1 on Hep G2 (Human hepatocellular carcinoma cell) cell lysate with Rabbit anti-SERPING1 antibody (HA752101) at 1/2,500 dilution.

Lysates/proteins at 15 µg/Lane.
Exposure time: 20 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752101, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 55 kDa
Observed band size: 95-105kDa
HA752101_2.jpg Fig2: This data was developed using HA752101, the same antibody clone in a different buffer formulation.
Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (HA752101) at 1/2,500 dilution.

Lane 1: Human serum lysate
Lane 2: Human urine lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752101, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 55 kDa
Observed band size: 95-105 kDa
HA752101_3.jpg Fig3: This data was developed using HA752101, the same antibody clone in a different buffer formulation.
Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (HA752101) at 1/2,500 dilution.

Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate

Lysates/proteins at 30 µg/Lane.
Exposure time: 20 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752101, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 55 kDa
Observed band size: 60-65 kDa
HA752101_4.jpg Fig4: This data was developed using HA752101, the same antibody clone in a different buffer formulation.
Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (HA752101) at 1/2,500 dilution.

Lane 1: Mouse plasma lysate
Lane 2: Rat plasma lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 46 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752101, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 55 kDa
Observed band size: 95-105 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.