| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH28-15 |
| Form: | Liquid |
| Storage condition: | Store at 2-8℃. Avoid freeze. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Positive control: | Hep G2 (Human hepatocellular carcinoma cell) cell lysates, Human serum lysate, Human urine lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Mouse plasma lysate, Rat plasma lysate. |
| Subcellular location: | Secreted. |
| Uniprot #: | SwissProt: P05155 Human | P97290 Mouse | Q6P734 Rat |
| Alternative names: | C1 esterase inhibitor C1 Inh C1 inhibiting factor C1 inhibitor C1-inhibiting factor C1IN C1Inh C1NH complement component 1 inhibitor esterase inhibitor HAE1 HAE2 IC1_HUMAN Plasma protease C1 inhibitor Serine (or cysteine) proteinase inhibitor clade G member 1 serine/cysteine proteinase inhibitor clade G member 1 Serpin G1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 SERPING1 |
|
Fig1:
This data was developed using HA752101, the same antibody clone in a different buffer formulation. Western blot analysis of SERPING1 on Hep G2 (Human hepatocellular carcinoma cell) cell lysate with Rabbit anti-SERPING1 antibody (HA752101) at 1/2,500 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA752101, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 95-105kDa |
|
Fig2:
This data was developed using HA752101, the same antibody clone in a different buffer formulation. Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (HA752101) at 1/2,500 dilution. Lane 1: Human serum lysate Lane 2: Human urine lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA752101, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 95-105 kDa |
|
Fig3:
This data was developed using HA752101, the same antibody clone in a different buffer formulation. Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (HA752101) at 1/2,500 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA752101, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 60-65 kDa |
|
Fig4:
This data was developed using HA752101, the same antibody clone in a different buffer formulation. Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (HA752101) at 1/2,500 dilution. Lane 1: Mouse plasma lysate Lane 2: Rat plasma lysate Lysates/proteins at 15 µg/Lane. Exposure time: 46 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA752101, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 95-105 kDa |