PEDF Recombinant Rabbit Monoclonal Antibody [PSH28-40]
cat.: HA752117
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Pig
Applications: WB, FC(Intra), IP
Clonality: Monoclonal
Clone number: PSH28-40
Form: Liquid
Storage condition: Store at 2-8℃. Avoid freeze.
Storage buffer: PBS (pH7.4).
Concentration: 0.5ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG
Immunogen: Recombinant protein within human PEDF aa 1-418.
Positive control: K-562 (Human chronic myelogenous leukemia cell) cell lysate, Y79 (Human retinoblastoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysates, NIH/3T3 (Mouse fibroblast) cell lysate, 3T3-L1 (Mouse embryonic fibroblast) cell lysate, C2C12 (Mouse myoblast) cell lysate, NRK (Rat kidney cell) cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Mouse stomach tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Human serum lysate, Mouse plasma lysate, Rat plasma lysate, Pig liver tissue lysate, Pig brain tissue lysate.
Subcellular location: Secreted, Melanosome.
Uniprot #: SwissProt: P36955 Human | P97298 Mouse
Entrez Gene: 287526 Rat
Alternative names: Cell proliferation-inducing gene 35 protein EPC 1 EPC-1 EPC1 OI12 OI6 PEDF PEDF_HUMAN PIG 35 PIG35 Pigment epithelium derived factor Pigment epithelium-derived factor Proliferation inducing protein 35 Serine (or cysteine) proteinase inhibitor serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 Serpin F1 Serpin family F member 1 Serpin peptidase inhibitor Serpin peptidase inhibitor clade F member 1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 SERPINF 1 Serpinf1
Images
HA752117_1.jpg Fig1: This data was developed using HA752117, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA752117) at 1/2,500 dilution.

Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 2: Y79 (Human retinoblastoma cell) cell lysate
Lane 3: MCF7 (Human breast cancer cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 2 minutes 6 seconds; ECL: K1801

MCF7 is a negative control (PMID: 16740777).

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752117, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 46 kDa
Observed band size: 50 kDa
HA752117_2.jpg Fig2: This data was developed using HA752117, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on Hep G2 (Human hepatocellular carcinoma cell) cell lysate with Rabbit anti-PEDF antibody (HA752117) at 1/2,500 dilution.

Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752117, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 46 kDa
Observed band size: 50 kDa
HA752117_3.jpg Fig3: This data was developed using HA752117, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA752117) at 1/2,500 dilution.

Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 2: 3T3-L1 (Mouse embryonic fibroblast) cell lysate
Lane 3: C2C12 (Mouse myoblast) cell lysate
Lane 4: NRK (Rat kidney cell) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 18 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752117, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 46 kDa
Observed band size: 50 kDa
HA752117_4.jpg Fig4: This data was developed using HA752117, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA752117) at 1/2,500 dilution.

Lane 1: Mouse liver tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse stomach tissue lysate
Lane 4: Rat liver tissue lysate
Lane 5: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute 2 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752117, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 46 kDa
Observed band size: 50 kDa
HA752117_5.jpg Fig5: This data was developed using HA752117, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA752117) at 1/2,500 dilution.

Lane 1: Human serum lysate
Lane 2: Mouse plasma lysate
Lane 3: Rat plasma lysate

Lysates/proteins at 10 µg/Lane.
Exposure time: 18 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752117, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 46 kDa
Observed band size: 50 kDa
HA752117_6.jpg Fig6: This data was developed using HA752117, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA752117) at 1/2,500 dilution.

Lane 1: Pig liver tissue lysate
Lane 2: Pig brain tissue lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute 2 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752117, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 46 kDa
Observed band size: 50 kDa
HA752117_7.jpg Fig7: This data was developed using HA752117, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry (Intra)

Species: Mouse
Sample: NIH/3T3 (Mouse fibroblasts)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.

Primary antibody: HA752117 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
HA752117_8.jpg Fig8: This data was developed using HA752117, the same antibody clone in a different buffer formulation.
Immunoprecipitation (IP)

PEDF was immunoprecipitated in 0.2 mg Hep G2 (Human hepatocellular carcinoma cell) cell lysate with HA752117 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA752117 at 1/2,500 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: Hep G2 cell lysate (input)
Lane 2: HA752117 IP in Hep G2 cell lysate
Lane 3: Rabbit IgG instead of HA752117 in Hep G2 cell lysate

Exposure time: 12 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature

Primary dilution: HA752117, 1/2,500 in primary antibody dilution buffer (K1803), 2 hours at room temperature

Predicted band size: 46 kDa
Observed band size: 50 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.