TGFBI Recombinant Rabbit Monoclonal Antibody [PSH28-78]
cat.: HA752124
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: PSH28-78
Form: Liquid
Storage condition: Store at 2-8℃. Avoid freeze.
Storage buffer: PBS (pH7.4).
Concentration: 0.5ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 75 kDa
Isotype: IgG
Immunogen: Recombinant protein within human TGFBI aa 1-683.
Positive control: A549 (Human lung adenocarcinoma cell) cell lysate, A549 treated with 5ng/mL hTGFβ1 for 48 hours add 300ng/mL BFA for last 16 hours cell lysate, NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysates.
Subcellular location: Secreted, extracellular space, extracellular matrix.
Uniprot #: SwissProt: Q15582 Human
Alternative names: RGD containing collagen associated protein AI181842 AI747162 Beta ig Beta ig h3 Beta ig-h3 BGH3_HUMAN Big h3 BIGH3 CDB1 CDG2 CDGG1 CSD CSD1 CSD2 CSD3 EBMD Kerato epithelin Kerato-epithelin LCD1 MGC150270 RGD CAP RGD-CAP RGD-containing collagen-associated protein TGFBI TGFBI transforming growth factor, beta induced, 68kDa Transforming growth factor beta induced protein ig h3 Transforming growth factor-beta-induced protein ig-h3
Images
HA752124_1.jpg Fig1: This data was developed using HA752124, the same antibody clone in a different buffer formulation.
Western blot analysis of TGFBI on different lysates with Rabbit anti-TGFBI antibody (HA752124) at 1/2,000 dilution.

Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 2: A549 treated with 5ng/mL hTGFβ1 for 48 hours add 300ng/mL BFA for last 16 hours cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801

The molecule weight observed is consistent with what has been described in the literature (PMID: 39068456, PMID: 37865162).

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752124, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 75 kDa
Observed band size: 70 kDa
HA752124_2.jpg Fig2: This data was developed using HA752124, the same antibody clone in a different buffer formulation.
Western blot analysis of TGFBI on NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysate with Rabbit anti-TGFBI antibody (HA752124) at 1/2,000 dilution.

Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801

The molecule weight observed is consistent with what has been described in the literature (PMID: 39068456, PMID: 37865162).

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752124, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 75 kDa
Observed band size: 70 kDa
HA752124_3.jpg Fig3: This data was developed using HA752124, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: A549 (Human lung adenocarcinoma cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA752124, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
HA752124_4.jpg Fig4: This data was developed using HA752124, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Squamous cell carcinoma of the lung
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752124, 1/8,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA752124_5.jpg Fig5: This data was developed using HA752124, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752124, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA752124_6.jpg Fig6: This data was developed using HA752124, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752124, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.