BubR1 Recombinant Rabbit Monoclonal Antibody [PSH29-05]
cat.: HA752138
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: PSH29-05
Form: Liquid
Storage condition: Store at 2-8℃. Avoid freeze.
Storage buffer: PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 120 kDa
Isotype: IgG
Immunogen: Recombinant protein within human BubR1 aa 1-200.
Positive control: HeLa (Human cervical adenocarcinoma cell) cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, Ramos (Human Burkitt's lymphoma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Mouse testis tissue lysate, Mouse thymus tissue lysate, Rat testis tissue lysate.
Subcellular location: Nucleus, Cytoplasm, Cytoskeleton.
Uniprot #: SwissProt: O60566 Human | Q9Z1S0 Mouse
Entrez Gene: 171576 Rat
Alternative names: Beta homolg of S. cerevisiae BUB 1 Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles BUB 1B BUB1 budding uninhibited by benzimidazoles 1 homolog beta Bub1A BUB1B BUB1B_HUMAN BUB1beta BUBR1 Budding Uninhibited by Benzimidazoles 1 beta Budding uninhibited by benzimidazoles 1 homolog beta (yeast) hBUBR1 MAD3/BUB1 related protein kinase MAD3/BUB1-related protein kinase MAD3L Mitotic checkpoint gene BUB1B Mitotic checkpoint kinase MAD3L Mitotic checkpoint serine/threonine protein kinase BUB1 beta Mitotic checkpoint serine/threonine-protein kinase BUB1 beta MVA1 OTTHUMP00000160319 Protein SSK1 SSK 1 SSK1
Images
HA752138_1.jpg Fig1: This data was developed using HA752138, the same antibody clone in a different buffer formulation.
Western blot analysis of BubR1 on different lysates with Rabbit anti-BubR1 antibody (HA752138) at 1/5,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: Raji (Human Burkitt's lymphoma cell) cell lysate
Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 4: Ramos (Human Burkitt's lymphoma cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752138, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 120 kDa
Observed band size: 120 kDa
HA752138_2.jpg Fig2: This data was developed using HA752138, the same antibody clone in a different buffer formulation.
Western blot analysis of BubR1 on different lysates with Rabbit anti-BubR1 antibody (HA752138) at 1/5,000 dilution.

Lane 1: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate (15 µg/Lane)
Lane 2: Mouse testis tissue lysate (20 µg/Lane)
Lane 3: Mouse thymus tissue lysate (20 µg/Lane)
Lane 4: Rat testis tissue lysate (20 µg/Lane)

Exposure time: 3 minutes; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752138, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 120 kDa
Observed band size: 120 kDa
HA752138_3.jpg Fig3: This data was developed using HA752138, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Testis
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752138, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA752138_4.jpg Fig4: This data was developed using HA752138, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Testis
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752138, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA752138_5.jpg Fig5: This data was developed using HA752138, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Thymus
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752138, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA752138_6.jpg Fig6: This data was developed using HA752138, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Thymus
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752138, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA752138_7.jpg Fig7: This data was developed using HA752138, the same antibody clone in a different buffer formulation.
Immunoprecipitation (IP)

BubR1 was immunoprecipitated in 0.2 mg Raji (Human Burkitt's lymphoma cell) cell lysate with HA752138 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA752138 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: Raji cell lysate (input)
Lane 2: HA752138 IP in Raji cell lysate
Lane 3: Rabbit IgG instead of HA752138 in Raji cell lysate

Exposure time: 3 minutes
Blocking: 5% NFDM/TBST, 1 hour at room temperature

Primary dilution: HA752138, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

Predicted band size: 120 kDa
Observed band size: 120 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.