TGF-beta Fibrosis Pathway Antibody Kit
cat.: HAK21004
| Product Type: | Antibody Sampler Kit |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Uniprot #: | SwissProt: P62736 Human | P62737 Mouse | P62738 Rat | P02452 Human | P11087 Mouse | P02454 Rat | Q15796 Human | Q15796 Human | Q62432 Mouse | O70436 Rat | Q15796 Human | Q62432 Mouse | O70436 Rat | P36222 Human | Q61362 Mouse | P37173 Human | P01137 Human | P04202 Mouse | P17246 Rat |
Images
|
Fig1:
Western blot analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody (ET1607-53) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: L6 cell lysate Lane 7: Mouse heart tissue lysate Lane 8: Mouse skin tissue lysate Lane 9: Rat heart tissue lysate Lane 10: Rat skin tissue lysate Lane 11: Rat smooth muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-53) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Smad2 on different lysates with Rabbit anti-Smad2 antibody (ET1604-22) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HT-29 cell lysate Lane 3: Jurkat cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 52 kDa Observed band size: 58 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-22) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of Phospho-Smad3(S423/S425) on different lysates with Rabbit anti-Phospho-Smad3(S423/S425) antibody (ET1609-41) at 1/2,000 dilution. Lane 1: A549 whole cell lysate Lane 2: A549 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate Lane 3: C2C12 whole cell lysate Lane 4: C2C12 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-41) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.