HAK21008

Glycolysis Antibody Kit

cat.: HAK21008

Product Type:	Antibody Sampler Kit
Species reactivity:	Human, Mouse, Rat
Applications:	WB

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Uniprot #:	SwissProt: P14618 Human \| P52480 Mouse \| P04406 Human \| P16858 Mouse \| P04797 Rat \| P08559 Human \| P35486 Mouse \| P26284 Rat \| P19367 Human \| P17710 Mouse \| P05708 Rat \| P52789 Human \| O08528 Mouse \| P27881 Rat \| P07195 Human \| P00338 Human \| P16125 Mouse \| P06151 Mouse \| P42123 Rat \| P04642 Rat \| Q01813 Human \| Q9WUA3 Mouse \| P47860 Rat

Images

Fig1: All lanes: Western blot analysis of PKM with anti-PKM antibody (R1603-5) at 1:500 dilution.

Lane 1: Wild-type MDA-MB-231 whole cell lysate.
Lane 2: PKM knockout MDA-MB-231 whole cell lysate.

R1603-5 was shown to specifically react with PKM in wild-type MDA-MB-231 cells. No band was observed when PKM knockout sample was tested. Wild-type and PKM knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1603-5, 1/500) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

Fig2: Western blot analysis of GAPDH on Hela cell lysates with Rabbit anti-GAPDH antibody (ET1601-4).

Hela cell lysates at 10 µg/Lane.

Predicted band size: 36 kDa
Observed band size: 36 kDa

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig3: Western blot analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody (ET1608-57) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HEK-293 cell lysate (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 1 minute 36 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-57) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.