HAK21009

Hippo Signaling Antibody Kit

cat.: HAK21009

Product Type:	Antibody Sampler Kit
Species reactivity:	Human
Applications:	WB

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Uniprot #:	SwissProt: Q9H8S9 Human \| Q7L9L4 Human \| Q921Y0 Mouse \| Q8BPB0 Mouse \| Q3T1J9 Rat \| P46937 Human \| P46938 Mouse \| Q2EJA0 Rat \| O95835 Human \| Q8BYR2 Mouse \| Q13188 Human \| Q9JI10 Mouse \| O54748 Rat \| Q13043 Human \| Q9JI11 Mouse \| P46937 Human \| P46938 Mouse \| Q2EJA0 Rat \| Q9GZV5 Human \| Q9EPK5 Mouse \| Q9H4B6 Human \| Q8VEB2 Mouse \| A4V8B4 Rat Entrez Gene: 360920 Rat \| 295062 Rat

Images

Fig1: Western blot analysis of Phospho-YAP1 (S127) on different lysates with Rabbit anti-Phospho-YAP1 (S127) antibody (ET1611-69) at 1/5,000 dilution.

Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lysates/proteins at 10 µg/Lane.
Lane 7: C6 whole cell lysate
Lane 8: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 9: C6 treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate

Predicted band size: 54 kDa
Observed band size: 70 kDa

Exposure time: 1 minute 6 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-69) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of SAV1 on different lysates with Rabbit anti-SAV1 antibody (HA722130) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

Lane 1: HCT 116 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 6: SW480 cell lysate (20 µg/Lane)
Lane 7: BxPC-3 cell lysate (20 µg/Lane)
Lane 8: 786-0 cell lysate (negative) (20 µg/Lane)
Lane 9: A549 cell lysate (20 µg/Lane)
Lane 10: NIH/3T3 cell lysate (20 µg/Lane)
Lane 11: PC-12 cell lysate (20 µg/Lane)
Lane 12: Mouse testis tissue lysate (40 µg/Lane)
Lane 13: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 3 minute4; ECL: Lane 1-13 (left): K1801; Lane 1-13 (right): K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722130) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.