Stat Antibody Sampler Kit
cat.: HAK21013
Product Type: Antibody Sampler Kit
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Uniprot #: SwissProt: P42224 Human | P42225 Mouse | P52630 Human | Q9WVL2 Mouse | P40763 Human | P42227 Mouse | P52631 Rat | Q14765 Human | P42228 Mouse | P42229 Human | P51692 Human | P42230 Mouse | P42232 Mouse | P52632 Rat | Q62771 Rat | P42226 Human | P52633 Mouse
Entrez Gene: 367264 Rat
Unigene: 24237 Rat
Images
HAK21013_1.jpg Fig1: Western blot analysis of STAT1 on different lysates with Rabbit anti-STAT1 antibody (ET1606-39) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate (10 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Lane 3: A431 cell lysate (10 µg/Lane)

Predicted band size: 87 kDa
Observed band size: 87 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-39) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HAK21013_2.jpg Fig2: Western blot analysis of STAT2 on different lysates with Rabbit anti-STAT2 antibody (ET1702-18) at 1/1,000 dilution.

Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: THP-1 cell lysate (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 98 kDa
Observed band size: 105 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-18) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HAK21013_3.jpg Fig3: Western blot analysis of STAT3 on different lysates with Rabbit anti-STAT3 antibody (ET1607-38) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 4: SW480 cell lysate (20 µg/Lane)
Lane 5: PC-3M cell lysate (20 µg/Lane)
Lane 6: RAW264.7 cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse brain tissue lysate (40 µg/Lane)
Lane 10: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 88 kDa
Observed band size: 88/50 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-38) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HAK21013_4.jpg Fig4: Western blot analysis of STAT4 on different lysates with Rabbit anti-STAT4 antibody (ET1701-42) at 1/1,000 dilution.

Lane 1: SiHa cell lysate, 20 µg/Lane
Lane 2: A549 cell lysate, 20 µg/Lane
Lane 3: HeLa cell lysate, 20 µg/Lane
Lane 4: MCF-7 cell lysate, 20 µg/Lane
Lane 5: SW480 cell lysate, 20 µg/Lane

Predicted band size: 86 kDa
Observed band size: 80 kDa

Exposure time: 6 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HAK21013_5.jpg Fig5: Western blot analysis of STAT 5A+B on different lysates with Rabbit anti-STAT 5A+B antibody (ET1701-45) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate, 20 µg/Lane
Lane 2: PC-12 cell lysate, 20 µg/Lane
Lane 3: HepG2 cell lysate, 20 µg/Lane
Lane 4: NIH/3T3 cell lysate, 20 µg/Lane
Lane 5: RH-35 cell lysate, 20 µg/Lane
Lane 6: HeLa cell lysate, 20 µg/Lane

Predicted band size: 90 kDa
Observed band size: 90 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-45) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HAK21013_6.jpg Fig6: Western blot analysis of STAT6 on different lysates with Rabbit anti-STAT6 antibody (ET1605-49) at 1/2,000 dilution.

Lane 1: THP-1 cell lysate, 20 µg/Lane
Lane 2: Jurkat cell lysate, 20 µg/Lane
Lane 3: Raji cell lysate, 20 µg/Lane
Lane 4: HeLa cell lysate, 20 µg/Lane
Lane 5: MDA-MB-231 cell lysate, 20 µg/Lane
Lane 6: NIH/3T3 cell lysate, 20 µg/Lane
Lane 7: RAW264.7 cell lysate, 20 µg/Lane

Predicted band size: 94 kDa
Observed band size: 100 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-49) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.