mTOR Pathway Antibody Sampler Kit
cat.: HAK21036
Product Type: Antibody Sampler Kit
Species reactivity: Human
Applications: WB
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Uniprot #: SwissProt: P42345 Human | Q9JLN9 Mouse | P42346 Rat | P42345 Human | Q9JLN9 Mouse | P42345 Human | Q9BVC4 Human | Q9DCJ1 Mouse | Q9Z2K5 Rat | Q8N122 Human | Q8K4Q0 Mouse | Q6R327 Human | Q6QI06 Mouse
Entrez Gene: 287871 Rat
Images
HAK21036_1.jpg Fig1: Western blot analysis of mTOR on different lysates with Rabbit anti-mTOR antibody (ET1608-5) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: C6 cell lysate
Lane 8: Mouse testis tissue lysate
Lane 9: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 289 kDa
Observed band size: 289 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-5) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
HAK21036_2.jpg Fig2: Western blot analysis of Phospho-mTOR (S2448) on different lysates with Mouse anti-Phospho-mTOR (S2448) antibody (HA600094) at 1/5,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 289 kDa
Observed band size: 289 kDa

Exposure time: 1 minute 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600094) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
HAK21036_3.jpg Fig3: Western blot analysis of Phospho-mTOR (S2481) on different lysates with Rabbit anti-Phospho-mTOR (S2481) antibody (HA721632) at 1/5,000 dilution.

Lane 1: HeLa whole cell lysate
Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 289 kDa
Observed band size: 289 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721632) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
HAK21036_4.jpg Fig4: Western blot analysis of MLST8 on different lysates with Rabbit anti-MLST8 antibody (HA500372) at 1/1,000 dilution.

Lane 1: C2C12 cell lysate (10 µg/Lane)
Lane 2: L6 cell lysate (10 µg/Lane)
Lane 3: Mouse brain tissue lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)

Predicted band size: 40 kDa
Observed band size: 36 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500372) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HAK21036_5.jpg Fig5: Western blot analysis of Raptor on different lysates with Rabbit anti-Raptor antibody (HA722028) at 1/2,000 dilution.

Lane 1: 293T cell lysate (30 µg/Lane)
Lane 2: K-562 cell lysate (30 µg/Lane)
Lane 3: Neuro-2a cell lysate (30 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 149 kDa
Observed band size: 149 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722028) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.