ER Homeostasis Antibody Sampler Kit
cat.: HAK21038
Product Type: Antibody Sampler Kit
Species reactivity: Human
Applications: WB
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Uniprot #: SwissProt: Q9H6L5 Human | Q8VE91 Mouse | Q5FVM3 Rat | Q9ULG6 Human | P17861 Human | O35426 Mouse | P18850 Human | F6VAN0 Mouse | G3V909 Rat | P05198 Human | Q6ZWX6 Mouse | P68101 Rat | Q9NZJ5 Human | P18848 Human | Q06507 Mouse | Q9ES19 Rat | O75460 Human | Q9EQY0 Mouse | P11021 Human | P20029 Mouse | P06761 Rat
Entrez Gene: 498013 Rat
Images
HAK21038_1.jpg Fig1: Western blot analysis of FAM134B on different lysates with Rabbit anti-FAM134B antibody (HA721752) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate (30 µg/Lane)
Lane 2: U-2 OS cell lysate (30 µg/Lane)
Lane 3: MCF7 cell lysate (30 µg/Lane)
Lane 4: HeLa cell lysate (30 µg/Lane)
Lane 5: HepG2 cell lysate (30 µg/Lane)
Lane 6: TT cell lysate (30 µg/Lane)
Lane 7: Jurkat cell lysate (30 µg/Lane)
Lane 8: NIH/3T3 cell lysate (30 µg/Lane)
Lane 9: C2C12 cell lysate (30 µg/Lane)
Lane 10: PC-12 cell lysate (30 µg/Lane)
Lane 11: C6 cell lysate (30 µg/Lane)
Lane 12: Mouse liver tissue lysate (30 µg/Lane)
Lane 13: Rat liver tissue lysate (30 µg/Lane)

Predicted band size: 55 kDa
Observed band size: 70 kDa

Exposure time: 6 minutes 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HAK21038_2.jpg Fig2: Western blot analysis of CCPG1 on different lysates with Rabbit anti-CCPG1 antibody (HA500520) at 1/1,000 dilution.

Lane 1: HCT116 cell lysate
Lane 2: A549 cell lysate
Lane 3: Hela cell lysate
Lane 4: THP-1 cell lysate
Lane 5: SW480 cell lysate
Lane 6: HEK-293 cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 87 kDa
Observed band size: 105 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500520) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HAK21038_3.jpg Fig3: Western blot analysis of XBP1 on different lysates with Rabbit anti-XBP1 antibody (ET1703-23) at 1/500 dilution.

Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 29 kDa
Observed band size: 40 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-23) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HAK21038_4.jpg Fig4: Western blot analysis of ATF6 on different lysates with Mouse anti-ATF6 antibody (HA601321) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Mouse kidney tissue lysate (40 µg/Lane)

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time: 1 minute 21 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601321) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
HAK21038_5.jpg Fig5: Western blot analysis of PERK on different lysates with Rabbit anti-PERK antibody (HA721510) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: A549 cell lysate (20 µg/Lane)
Lane 6: U-87 MG cell lysate (20 µg/Lane)
Lane 7: K-562 cell lysate (16 µg/Lane)

Predicted band size: 125 kDa
Observed band size: 140 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721510) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HAK21038_6.jpg Fig6: Western blot analysis of Phospho-EIF2S1 (S51) on different lysates with Rabbit anti-Phospho-EIF2S1 (S51) antibody (ET1603-14) at 1/2,000 dilution.

Lane 1: HeLa whole cell lysate (15 µg/Lane)
Lane 2: HeLa treated with 50nM Calyculin A for 3 hours whole cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 whole cell lysate (15 µg/Lane)
Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate (15 µg/Lane)
Lane 5: C6 whole cell lysate (20 µg/Lane)
Lane 6: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane)

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: Lane 1-4: 2 minutes; Lane 5-6: 23 seconds；

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-14) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
HAK21038_7.jpg Fig7: Western blot analysis of ATF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: PC-12 cell lysate
Lane 3: HL-60 cell lysate
Lane 4: K562 cell lysate
Lane 5: human lung carcinoma tissue lysate
HAK21038_8.jpg Fig8: Western blot analysis of GRP78 / BIP on different lysates with Mouse anti-GRP78 / BIP antibody (HA601076) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate (15 µg/Lane)
Lane 2: HepG2 cell lysate (15 µg/Lane)
Lane 3: Mouse brain tissue lysate (30 µg/Lane)
Lane 4: U-87 MG cell lysate (30 µg/Lane)
Lane 5: RAW264.7 cell lysate (30 µg/Lane)
Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 µg/Lane)
Lane 7: Mouse liver tissue lysate (30 µg/Lane)
Lane 8: Rat liver tissue lysate (30 µg/Lane)
Lane 9: Rat pancreas tissue lysate (30 µg/Lane)

Predicted band size: 72 kDa
Observed band size: 72 kDa

Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601076) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.