Cell Cycle Regulation Antibody Sampler Kit
cat.: HAK21039
Product Type: Antibody Sampler Kit
Species reactivity: Human
Applications: WB
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Uniprot #: SwissProt: P24941 Human | P97377 Mouse | Q63699 Rat | P46527 Human | P46414 Mouse | P24385 Human | P25322 Mouse | P39948 Rat | Q00534 Human | P30281 Human | P30282 Mouse | P48961 Rat | P38936 Human | P11802 Human | P30285 Mouse | P35426 Rat | P42773 Human | Q60772 Mouse
Entrez Gene: 54238 Rat
Unigene: 29897 Rat
Images
HAK21039_1.jpg Fig1: Western blot analysis of Cdk2 on different lysates with Rabbit anti-Cdk2 antibody (ET1602-6) at 1/500 dilution.

Lane 1: A549 cell lysate, 10 µg/Lane
Lane 2: F9 cell lysate, 10 µg/Lane

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-6) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HAK21039_2.jpg Fig2: Western blot analysis of p27 KIP 1 on different lysates with Rabbit anti-p27 KIP 1 antibody (ET1608-61) at 1/2,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 22 kDa
Observed band size: 27 kDa

Exposure time: 3 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-61) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
HAK21039_3.jpg Fig3: Western blot analysis of Cyclin D1 on different lysates with Rabbit anti-Cyclin D1 antibody (ET1601-31) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.

Lane 1: MCF7 cell lysate, 20 µg/Lane
Lane 2: K-562 cell lysate (negative), 20 µg/Lane
Lane 3: A431 cell lysate, 20 µg/Lane
Lane 4: Neuro-2a cell lysate, 20 µg/Lane
Lane 5: NIH/3T3 cell lysate, 20 µg/Lane
Lane 6: C6 cell lysate, 20 µg/Lane
Lane 7: SH-SY5Y cell lysate, 20 µg/Lane

Predicted band size: 34 kDa
Observed band size: 35 kDa

Exposure time: 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-31) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HAK21039_4.jpg Fig4: Western blot analysis of Cdk4 on different lysates with Rabbit anti-Cdk4 antibody (ET1612-23) at 1/1,000 dilution.

Lane 1: Hela cell lysate, 10 µg/Lane
Lane 2: MCF-7 cell lysate, 10 µg/Lane
Lane 3: K562 cell lysate, 10 µg/Lane
Lane 4: SKOV-3 cell lysate, 10 µg/Lane

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-23) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HAK21039_5.jpg Fig5: Western blot analysis of p18 INK4c on different lysates with Rabbit anti-p18 INK4c antibody (HA721960) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 6: Rat skeletal muscle tissue lysate (40 µg/Lane)

Predicted band size: 18 kDa
Observed band size: 18 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721960) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.