Mitochondrial Marker Antibody Sampler Kit
cat.: HAK21041
| Product Type: | Antibody Sampler Kit |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Uniprot #: | SwissProt: P13073 Human | P19783 Mouse | P10888 Rat | P99999 Human | P62897 Mouse | P62898 Rat | P10809 Human | P63038 Mouse | P63039 Rat | P35232 Human | P67778 Mouse | P67779 Rat | Q7T1D8 Zebrafish | P08559 Human | P35486 Mouse | P26284 Rat | P31040 Human | Q8K2B3 Mouse | Q920L2 Rat | P00441 Human | P08228 Mouse | P07632 Rat | P21796 Human | Q60932 Mouse | Q9Z2L0 Rat |
Images
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Fig1:
Western blot analysis of COX IV on different lysates with Rabbit anti-COX IV antibody (ET1701-63) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Mouse heart tissue lysate (30 µg/Lane) Lane 4: Rat heart tissue lysate (30 µg/Lane) Predicted band size: 20 kDa Observed band size: 15 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-63) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Cytochrome C on different lysates with Rabbit anti-Cytochrome C antibody (ET1610-60) at 1/2,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: C6 cell lysate (15 µg/Lane) Lane 4: Human kidney tissue lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 12 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-60) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of Hsp60 on different lysates with Rabbit anti-Hsp60 antibody (ET1609-45) at 1/100,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: SW480 cell lysate Lane 4: Jurkat cell lysate Lane 5: PANC-1 cell lysate Lane 6: F9 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-45) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Western blot analysis of Prohibitin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: Mouse kidney tissue lysate Lane 3: 293 cell lysate Lane 4: HepG2 cell lysate |
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Fig5:
Western blot analysis of Superoxide Dismutase 1 on different lysates with Rabbit anti-Superoxide Dismutase 1 antibody (ET1702-36) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Mouse hippocampus tissue lysate (40 µg/Lane) Lane 6: Mouse stomach tissue lysate (40 µg/Lane) Lane 7: Mouse smooth muscle tissue lysate (40 µg/Lane) Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig6:
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (ET1601-20) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: MDA-MB-231 cell lysate, 15 µg/Lane Lane 2: HeLa cell lysate, 15 µg/Lane Lane 3: K-562 cell lysate, 15 µg/Lane Lane 4: NIH/3T3 cell lysate, 15 µg/Lane Lane 5: PC-12 cell lysate, 15 µg/Lane Lane 6: Mouse kidney tissue lysate, 15 µg/Lane Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-20) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.