Fatty Acid and Lipid Metabolism Antibody Sampler Kit
cat.: HAK21048
Product Type: Antibody Sampler Kit
Species reactivity: Human, Mouse
Applications: WB
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Uniprot #: SwissProt: Q9NR19 Human | Q8BK97 Mouse | Q9QXG4 Mouse | D3ZFF9 Rat | D3ZUL6 Rat | Q13085 Human | Q5SWU9 Mouse | P11497 Rat | P53396 Human | Q91V92 Mouse | P16638 Rat | P49327 Human | P19096 Mouse | P12785 Rat | Q14693 Human | P33121 Human | P41216 Mouse | P18163 Rat | Q13085 Human | Q5SWU9 Mouse | P11497 Rat
Images
HAK21048_1.jpg Fig1: Western blot analysis of Acetyl CoA synthetase on different lysates with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: L6 cell lysate (20 µg/Lane)
Lane 3: COS-1 cell lysate (20 µg/Lane)

Predicted band size: 79 kDa
Observed band size: 79 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HAK21048_2.jpg Fig2: Western blot analysis of Acetyl CoA Carboxylase 1 (ACC1) on different lysates with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (ET1609-77) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: C2C12 cell lysate (20 µg/Lane)
Lane 6: RAW264.7 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)

Predicted band size: 266 kDa
Observed band size: 250/266 kDa

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-77) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HAK21048_3.jpg Fig3: Western blot analysis of ATP citrate lyase with anti-ATP citrate lyase antibody [ST51-07] (ET1609-37) at 1/1,000 dilution.
Lane 1: Wild-type A549 whole cell lysate (20 µg).
Lane 2: ATP citrate lyase knockout A549 whole cell lysate (20 µg).

ET1609-37 was shown to specifically react with ATP citrate lyase in wild-type A549 cells. No band was observed when ATP citrate lyase knockout sample was tested. Wild-type and ATP citrate lyase knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1609-37, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HAK21048_4.jpg Fig4: Western blot analysis of Fatty Acid Synthase on different lysates with Rabbit anti-Fatty Acid Synthase antibody (ET1701-91) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A549 cell lysate
Lane 4: C2C12 cell lysate
Lane 5: L-929 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 273 kDa
Observed band size: 273 kDa

Exposure time: 1 minute 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-91) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HAK21048_5.jpg Fig5: Western blot analysis of ACSL1 on different lysates with Mouse anti-ACSL1 antibody (HA601112) at 1/1,000 dilution.

Lane 1: Raji cell lysate (10 µg/Lane)
Lane 2: HepG2 cell lysate (10 µg/Lane)
Lane 3: LO2 cell lysate (10 µg/Lane)
Lane 4: A431 cell lysate (10 µg/Lane)

Predicted band size: 78 kDa
Observed band size: 78 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601112) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
HAK21048_6.jpg Fig6: Western blot analysis of Phospho-Acetyl Coenzyme A Carboxylase (S79) on different lysates with Rabbit anti-Phospho-Acetyl Coenzyme A Carboxylase (S79) antibody (HA721714) at 1/2,000 dilution.

Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 0.5μM Oligomycin for 30 minutes cell lysate
Lane 3: NIH/3T3 treated with 0.5μM Oligomycin for 30 minutes then treated with λpp for 1 hour cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 265 kDa
Observed band size: 265 kDa

Exposure time: 1 minutes 14 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721714) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.