Integrin Antibody Sampler Kit
cat.: HAK21053
| Product Type: | Antibody Sampler Kit |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Uniprot #: | SwissProt: P08648 Human | P11688 Mouse | P06756 Human | P43406 Mouse | P16144 Human | A2A863 Mouse | P13612 Human | Q00651 Mouse | P05556 Human | P09055 Mouse | P49134 Rat | P05106 Human | O54890 Mouse | P18084 Human Entrez Gene: 296456 Rat | 311144 Rat | 29302 Rat Unigene: 100796 Rat |
Images
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Fig1:
All lanes: Western blot analysis of Integrin alpha V with anti-Integrin alpha V antibody (ET1610-15) at 1/500 dilution. Lane 1: Wild-type A549 whole cell lysate. Lane 2/3: Integrin alpha V knockdown A549 whole cell lysate. ET1610-15 was shown to specifically react with Integrin alpha V in Wild-type A549 cells. Weakened bands were observed when Integrin alpha V knockdown samples were tested. Wild-type and Integrin alpha V knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Integrin alpha V antibody (ET1610-15, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.