HAK21057

Traumatic Brain Injury Biomarker Antibody Sampler Kit

cat.: HAK21057

Product Type:	Antibody Sampler Kit
Species reactivity:	Human, Mouse, Rat
Applications:	WB

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Uniprot #:	SwissProt: P14136 Human \| P03995 Mouse \| P47819 Rat \| P04271 Human \| P50114 Mouse \| P04631 Rat \| P09936 Human \| Q9R0P9 Mouse \| Q00981 Rat \| P07196 Human \| P08551 Mouse \| P19527 Rat \| P09104 Human \| P17183 Mouse \| P07323 Rat \| P02686 Human \| P04370 Mouse \| P02688 Rat \| P78352 Human \| Q62108 Mouse \| P31016 Rat \| P10636 Human \| P10637 Mouse \| P19332 Rat

Images

Fig1: Western blot analysis of PGP9.5 on different lysates with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/2,000 dilution.

Lane 1: A-172 cell lysate (20 µg/Lane)
Lane 2: SHG-44 cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: NCI-H1299 cell lysate (20 µg/Lane)
Lane 6: A549 cell lysate (20 µg/Lane)
Lane 7: 293T cell lysate (20 µg/Lane)
Lane 8: Neuro-2a cell lysate (20 µg/Lane)
Lane 9: C6 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse brain tissue lysate (30 µg/Lane)
Lane 12: Rat brain tissue lysate (30 µg/Lane)
Lane 13: LNCaP cell lysate (negative) (20 µg/Lane)
Lane 14: K-562 cell lysate (negative) (20 µg/Lane)

Predicted band size: 25 kDa
Observed band size: 25 kDa

Exposure time: 7 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of PSD95 on different lysates with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse hippocampus tissue lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat hippocampus tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 80 kDa
Observed band size: 75-100 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-20) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.