Matrix Remodeling Antibody Sampler Kit
cat.: HAK21072
| Product Type: | Antibody Sampler Kit |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Uniprot #: | SwissProt: P01033 Human | P16035 Human | P35625 Human | P48032 Rat | P08253 Human | P33434 Mouse | P33436 Rat | P08254 Human | P28862 Mouse | P03957 Rat | P09237 Human | P14780 Human | P41245 Mouse | P50282 Rat | P50281 Human | P53690 Mouse | Q10739 Rat |
Images
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Fig1:
Western blot analysis of MMP-14 on different lysates with Rabbit anti-MMP-14 antibody (ET1606-48) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: MEF cell lysate Lane 3: L6 cell lysate Lane 4: Mouse lung tissue lysate Lane 5: Rat lung tissue lysate Lysates/proteins at 20/40 µg/Lane. Predicted band size: 66 kDa Observed band size: 55 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of MMP-9 on different lysates with Rabbit anti-MMP-9 antibody (ET1704-69) at 1/5,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 200nM TPA for 10 minutes whole cell lysate Lane 3: Rat lung tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 78 kDa Observed band size: 92 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-69) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.